SMPD1 Antibody (YA8000)

製品番号: HY-P88316: データシート User Guide for Antibodies
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SMPD1 Antibody (YA8000) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SMPD1.

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容量	価格（税別）	在庫状況
20 μL	$115	見積もりとリードタイムを求めるのご確認
50 μL	$185	見積もりとリードタイムを求めるのご確認
100 μL	$300	見積もりとリードタイムを求めるのご確認

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明
参考文献

製品説明

SMPD1 Antibody (YA8000) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SMPD1.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human

SwissProt ID

P17405

Immunogen

Human recombinant protein fragment corresponding to amino acids 62-341 of human SMPD1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	純度	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Converts sphingomyelin to ceramide (PubMed:12563314, PubMed:1840600, PubMed:18815062, PubMed:25339683, PubMed:25920558, PubMed:27659707, PubMed:33163980). Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly (PubMed:20807762, PubMed:21098024, PubMed:9660788). However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form (PubMed:12563314, PubMed:20530211, PubMed:20807762, PubMed:22573858, PubMed:9393854)|In the lysosomes, converts sphingomyelin to ceramide (PubMed:20807762, PubMed:21098024). Plays an important role in the export of cholesterol from the intraendolysosomal membranes (PubMed:25339683). Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol (PubMed:25339683). Modulates stress-induced apoptosis through the production of ceramide (PubMed:8706124)|When secreted, modulates cell signaling with its ability to reorganize the plasma membrane by converting sphingomyelin to ceramide (PubMed:12563314, PubMed:17303575, PubMed:20807762). Secreted form is increased in response to stress and inflammatory mediators such as IL1B, IFNG or TNF as well as upon infection with bacteria and viruses (PubMed:12563314, PubMed:20807762, PubMed:9393854). Produces the release of ceramide in the outer leaflet of the plasma membrane playing a central role in host defense (PubMed:12563314, PubMed:20807762, PubMed:9393854). Ceramide reorganizes these rafts into larger signaling platforms that are required to internalize P.aeruginosa, induce apoptosis and regulate the cytokine response in infected cells (PubMed:12563314). In wounded cells, the lysosomal form is released extracellularly in the presence of Ca(2+) and promotes endocytosis and plasma membrane repair (PubMed:20530211)|This form is generated following cleavage by CASP7 in the extracellular milieu in response to bacterial infection (PubMed:21157428). It shows increased ability to convert sphingomyelin to ceramide and promotes plasma membrane repair (By similarity). Plasma membrane repair by ceramide counteracts the action of gasdermin-D (GSDMD) perforin (PRF1) pores that are formed in response to bacterial infection (By similarity)|(Microbial infection) Secretion is activated by bacteria such as P.aeruginosa, N.gonorrhoeae and others, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection|(Microbial infection) Secretion is activated by human coronaviruses SARS-CoV and SARS-CoV-2 as well as Zaire ebolavirus, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection|Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity|Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity

Subcellular Localization：Lysosome,Lipid droplet,Secreted,Secreted, extracellular space

Isoforms & Post-Translational Modification：P17405 has four isomers: P17405-1: 69936 Da (predicted); P17405-2: 65074 Da (predicted); P17405-3: 63695 Da (predicted); P17405-4: 69808 Da (predicted).
Proteolytically processed (PubMed:21098024, PubMed:9030779)丨This form is generated following cleavage by CASP7 in the extracellular milieu (PubMed:21157428)丨Both lysosomal and secreted forms are glycosylated but they show a differential pattern of glycosylation丨Phosphorylated at Ser-510 by PRKCD upon stress stimuli

Subunit：Monomer

Synonyms

ASM; ASMASE; NPD

ドキュメンテーション

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/u-251-cell.html [Content Brief]