SRY Antibody (YA8100)

Cat. No.: HY-P88416: Data Sheet User Guide for Antibodies
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SRY Antibody (YA8100) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SRY.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SRY Antibody (YA8100) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SRY.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 23.7 kDa

Species Reactivity

Human

SwissProt ID

Q05066

Immunogen

Full length human recombinant protein of human SRY produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcriptional regulator that controls a genetic switch in male development (PubMed:11563911). It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (PubMed:16414182, PubMed:16996051). Involved in different aspects of gene regulation including promoter activation or repression (PubMed:9525897). Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3' (PubMed:11563911, PubMed:1425584, PubMed:15170344, PubMed:8159753, PubMed:8265659). SRY HMG box recognizes DNA by partial intercalation in the minor groove and promotes DNA bending (PubMed:11563911, PubMed:1425584, PubMed:15170344, PubMed:16762365, PubMed:8159753, PubMed:8265659). Also involved in pre-mRNA splicing (PubMed:11818535). In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity)

Subcellular Localization：Nucleus speckle,Cytoplasm,Nucleus

Isoforms & Post-Translational Modification：Q05066: 204 amino acids, molecular weight 23884 Da.

Subunit：Interacts with CALM, EP300, HDAC3, KPNB1, ZNF208 isoform KRAB-O, PARP1, SLC9A3R2 and WT1 (PubMed:12871148, PubMed:12970737, PubMed:15297880, PubMed:15469996, PubMed:15746192, PubMed:16904257, PubMed:9054412)

Synonyms

SRXX1; SRXY1; TDF; TDY

Documentation

User Guide for Antibodies (1077 KB)