alpha Synuclein Antibody (YA5072)
(Synonyms: SNCA; NACP; PARK1; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP)alpha Synuclein Antibody (YA5072) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to alpha Synuclein.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P; IHC-F; IF-Tissue
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Reactivity :
Human
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|
| Dilution Ratio | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
alpha Synuclein Antibody (YA5072) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to alpha Synuclein.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
KLH conjugated synthetic peptide derived from human Alpha-Synuclein. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein G
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
alpha Synuclein is a Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity[1][2][3][4].
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Subcellular Localization
Cytoplasm; Membrane; Nucleus; Synapse; Secreted; Cell projection, axon
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Expression
Tissue_specificity:Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain. -
Isoforms & Post-Translational Modification
P37840 has 3 isomers: P37840-1: 14460 Da (predicted); P37840-2: 11372 Da (predicted); P37840-3: 13109 Da (predicted).
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress;Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers;Ubiquitinated. The predominant conjugate is the diubiquitinated form;Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure -
Subunit
Soluble monomer. Homotetramer (PubMed:21841800). A dynamic intracellular population of tetramers and monomers coexists normally and the tetramer plays an essential role in maintaining homeostasis (PubMed:21841800).
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SwissProt ID
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Synonyms
SNCA; NACP; PARK1; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
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