Telomeric Repeat Binding Factor 2 Antibody (YA4288)(PBS only)

Cat. No.: HY-P84591A: Ficha de datos User Guide for Antibodies
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Telomeric Repeat Binding Factor 2 Antibody (YA4288) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Telomeric Repeat Binding Factor 2.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

Telomeric Repeat Binding Factor 2 Antibody (YA4288) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Telomeric Repeat Binding Factor 2.

Host

Mouse

Peso molecular

Predicted band size: 60 kDa;

Observed band size: 66 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q15554

Gene ID

7014 [NCBI]

Immunogen

Purified recombinant fragment of human TERF2 (AA: 324-500) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes (PubMed:15608617, PubMed:16166375, PubMed:20655466, PubMed:28216226, PubMed:9326950, PubMed:9326951, PubMed:9476899). In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo (PubMed:16166375, PubMed:20655466). Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection (PubMed:16166375). Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways (PubMed:16166375). Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair (PubMed:20655466). Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo (PubMed:20655466, PubMed:28216226). Preferentially binds to positive supercoiled DNA (PubMed:15608617, PubMed:20655466). Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology (PubMed:20655466). Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length (By similarity)

Subcellular Localization：Nucleus; Chromosome, telomere

Expression：
Tissue_specificity:It is widely distributed. It is highly expressed in the spleen, thymus, prostate, uterus, testes, small intestine, colon, and peripheral blood leukocytes.

Isoforms & Post-Translational Modification：Q15554 has 2 isomers: Q15554-3: 59594 Da (predicted); Q15554-4: 32298 Da (predicted).
Phosphorylated upon DNA damage, most probably by ATM. Phosphorylated TERF2 is not bound to telomeric DNA, and rapidly localizes to damage sites;Methylated by PRMT1 at multiple arginines within the N-terminal Arg-rich region. Methylation may control association with telomeres

Subunit：Homodimer (PubMed:15608617). Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1 (PubMed:15316005, PubMed:15383534, PubMed:15608617, PubMed:18202258). Interacts with NBN; interaction takes place with unphosphorylated NBN during G1 phase and prevents to prevent ATM activation and non-homologous end joining repair (PubMed:10888888, PubMed:28216226). Interacts with SLX4/BTBD12 (PubMed:19596235). Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct (PubMed:16606622, PubMed:16730175, PubMed:16730176, PubMed:18468965, PubMed:18593705, PubMed:20655466)

Synonyms

TRF2; TRBF2

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/dotc2-4510-cell.html