TUBA1A Antibody (YA8250)

Cat. No.: HY-P88566: Data Sheet User Guide for Antibodies
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TUBA1A Antibody (YA8250) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to TUBA1A.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TUBA1A Antibody (YA8250) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to TUBA1A.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 50 kDa

Species Reactivity

Human, Dog, Rat, Monkey, Mouse

SwissProt ID

Q71U36

Immunogen

Full length human recombinant protein of human TUBA1A produced in HEK293T cell

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin

Subcellular Localization：Cytoplasm, cytoskeleton,Cytoplasm, cytoskeleton, flagellum axoneme

Expression：
Tissue_Specificity: Expressed at a high level in fetal brain

Isoforms & Post-Translational Modification：Q71U36 has two isomers: Q71U36-1: 50136 Da (predicted); Q71U36-2: 46297 Da (predicted).
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866)丨Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human丨Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen丨Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability丨Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution丨Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (MATCAP1/KIAA0895L, VASH1 or VASH2) and tubulin tyrosine ligase (TTL), respectively丨Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1丨Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662)

Subunit：Dimer of alpha and beta chains

Synonyms

B-ALPHA-1; LIS3; TUBA3

Documentation

User Guide for Antibodies (1077 KB)