USP44 Antibody (YA8687)

Cat. No.: HY-P89003: Data Sheet User Guide for Antibodies
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USP44 Antibody (YA8687) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to USP44.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

USP44 Antibody (YA8687) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to USP44.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 81 kDa

Species Reactivity

Human

SwissProt ID

Q9H0E7

Immunogen

Human recombinant protein fragment corresponding to amino acids 420-712 of human USP44 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C) (PubMed:17443180). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C (PubMed:17443180). Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint (PubMed:17443180). Also promotes the deubiquitination of histone H2A and H2B (PubMed:23615962, PubMed:27880911). Recruited to RNF8/RNF168-ubiquitinated chromatin surrounding double stranded breaks (DSBs), promotes hydrolysis of such ubiquitin conjugates, thus negatively regulating protein recruitment to damaged chromatin (PubMed:23615962). Participates in nucleotide excision repair (NER) pathway by deubiquitinating DDB2 to prevent its premature degradation so it can remain on damaged chromatin (By similarity). Promotes FOXP3 stabilization through 'Lys-48'-linked deubiquitination leading to increased stability and increased regulatory T-cell lineage stability (PubMed:32644293). Also plays a positive role in innate immune response to DNA viruses by deubiquitinating STING1, selectively removing its 'Lys-48'-linked polyubiquitin chains and stabilizing it (PubMed:31968013)

Subcellular Localization：Nucleus,Cytoplasm

Expression：
Tissue_Specificity: Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia
Induction: Transcriptionally regulated by POU5F1/OCT4 in embryonic stem cells and embryonal carcinoma cells. Induced by TGF-beta during regulatory T-cells differentiation (PubMed:32644293)

Isoforms & Post-Translational Modification：Q9H0E7: 712 amino acids, molecular weight 81185 Da.

Subunit：Interacts with the N-CoR components TBL1X and TBL1XR1

Synonyms

DKFZp434D0127; FLJ14528

Documentation

User Guide for Antibodies (1077 KB)