USP44 Antibody (YA8687)
(Synonyms: DKFZp434D0127; FLJ14528)USP44 Antibody (YA8687) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to USP44.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
IHC-P
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Reactivity :
Human
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:150-500 |
Product Details
USP44 Antibody (YA8687) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to USP44.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 81 kDa
Human recombinant protein fragment corresponding to amino acids 420-712 of human USP44 produced in E.coli.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Liquid
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
USP44 is a Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Also promotes the deubiquitination of histone H2A and H2B. Recruited to RNF8/RNF168-ubiquitinated chromatin surrounding double stranded breaks (DSBs), promotes hydrolysis of such ubiquitin conjugates, thus negatively regulating protein recruitment to damaged chromatin. Participates in nucleotide excision repair (NER) pathway by deubiquitinating DDB2 to prevent its premature degradation so it can remain on damaged chromatin. Promotes FOXP3 stabilization through 'Lys-48'-linked deubiquitination leading to increased stability and increased regulatory T-cell lineage stability. Also plays a positive role in innate immune response to DNA viruses by deubiquitinating STING1, selectively removing its 'Lys-48'-linked polyubiquitin chains and stabilizing it[1][2][3][4][5].
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Subcellular Localization
Nucleus,Cytoplasm
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Expression
Tissue_Specificity: Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia
Induction: Transcriptionally regulated by POU5F1/OCT4 in embryonic stem cells and embryonal carcinoma cells. Induced by TGF-beta during regulatory T-cells differentiation (PubMed:32644293) -
Isoforms & Post-Translational Modification
Q9H0E7: 712 amino acids, molecular weight 81185 Da.
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Subunit
Interacts with the N-CoR components TBL1X and TBL1XR1
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SwissProt ID
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Synonyms
DKFZp434D0127; FLJ14528
Documentation
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