WIBG Antibody (YA8409)

Cat. No.: HY-P88725: Data Sheet User Guide for Antibodies
FAQs
Technical Support

WIBG Antibody (YA8409) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WIBG.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

WIBG Antibody (YA8409) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WIBG.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 22.5 kDa

Species Reactivity

Human

SwissProt ID

Q9BRP8

Immunogen

Full length human recombinant protein of human WIBG produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmark for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as an EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC

Subcellular Localization：Cytoplasm,Nucleus, nucleolus,Nucleus, nucleoplasm

Isoforms & Post-Translational Modification：Q9BRP8 has two isomers: Q9BRP8-1: 22656 Da (predicted); Q9BRP8-2: 22705 Da (predicted).

Subunit：Interacts (via N-terminus) with MAGOH and RBM8A; the interaction is direct

Synonyms

PYM; WIBG

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hct-116-cell.html [Content Brief]