beta-1,4-Galactosyltransferase 1 (Y285L)
Based on 1 Customer Validation
beta-1,4-Galactosyltransferase 1 (Y285L) can enzymatic synthesis of the LacdiNAc motif. beta-1,4-Galactosyltransferase 1 (Y285L) can transfer of GalNAc from UDP-GalNAc.
For research use only. We do not sell to patients.
- Molecular Weight:39.7 kDa
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Product Information
This product is expressed in recombinant HEK293 cells, C-terminal His Tagged, molecular weight 39.7 kDa, storage buffer components are 20 mM Tris-HCl (PH7.4), 200 mM NaCl, 20% Glycerin, can effectively transfer GalNAc or GalNAz and other derivatives to the terminal GlcNAc residue, the latter can be used in antibody-drug conjugates (ADC) as targeted cancer therapy, etc.
Instructions (can be adjusted according to experimental conditions)
1. Reaction system:
100 mM Tris-HCl (pH 7.5), 6 mM MnCl2;
2. Reaction reagents:
1) UDP-GalNAz: antibody (equivalent ratio) = 300:1;
2) Enzyme addition amount: enzyme/antibody = 5%;
3) Prepare the reaction solution according to the above conditions, react at 37°C for 48 hours; then place at -15°C to -25°C to stop the reaction and perform sample testing.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
2.4.1-
Technical Parameters
-
Source
HEK293 cells
-
Tag
C-terminal His Tagged
Chemical Information
-
Appearance Liquid
-
Molecular Weight 39.7 kDa
-
Color Colorless to light yellow
-
SMILES
[beta-1,4-Galactosyltransferase 1 (Y285L)]
-
Synonyms
B4GALT1 (Y285L)
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (265 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
beta-1,4-Galactosyltransferase 1 (Y285L) Related Classifications
HY-E70285 Related Classifications
Powered by Bioz