FUT8 Protein, Human (sf9, His)

FUT8 protein serves as a catalyst in the glycosylation process, specifically adding fucose in an alpha 1-6 linkage to the first GlcNAc residue located adjacent to peptide chains in N-glycans. This enzymatic activity plays a crucial role in modifying glycoproteins, contributing to the structural diversity and functional properties of N-glycans. By facilitating the addition of fucose in a specific linkage, FUT8 participates in the intricate molecular landscape of glycosylation, influencing various cellular processes and interactions involving glycoproteins. FUT8 Protein, Human (sf9, His) is the recombinant human-derived FUT8 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of FUT8 Protein, Human (sf9, His) is 508 a.a., with molecular weight of ~55 kDa.

FUT8 protein serves as a catalyst in the glycosylation process, specifically adding fucose in an alpha 1-6 linkage to the first GlcNAc residue located adjacent to peptide chains in N-glycans. This enzymatic activity plays a crucial role in modifying glycoproteins, contributing to the structural diversity and functional properties of N-glycans. By facilitating the addition of fucose in a specific linkage, FUT8 participates in the intricate molecular landscape of glycosylation, influencing various cellular processes and interactions involving glycoproteins.

Measured by its ability to hydrolyze the donor substrate GDP fucose and the specific activity is >0.75 pmoles/min/μg.

Human

Sf9 insect cells

C-His

Q9BYC5 (R68-K575)

2530  [NCBI]

Approximately 55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.