FUT8 Protein, Human (sf9, His)
Based on 1 Customer Validation
The FUT8 protein acts as a catalyst during glycosylation, specifically adding fucose in an α1-6 linkage to the first GlcNAc residue located near the N-glycan peptide chain. This enzymatic activity plays a crucial role in glycoprotein modification, contributing to the structural diversity and functional properties of N-glycans. FUT8 Protein, Human (sf9, His) is the recombinant human-derived FUT8 protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The FUT8 protein acts as a catalyst during glycosylation, specifically adding fucose in an α1-6 linkage to the first GlcNAc residue located near the N-glycan peptide chain. This enzymatic activity plays a crucial role in glycoprotein modification, contributing to the structural diversity and functional properties of N-glycans. FUT8 Protein, Human (sf9, His) is the recombinant human-derived FUT8 protein, expressed by Sf9 insect cells , with C-His labeled tag.
FUT8, or alpha-1,6-fucosyltransferase 8, is an enzyme that plays a key role in glycosylation processes by catalyzing the addition of fucose in an alpha 1-6 linkage to the initial GlcNAc residue adjacent to peptide chains in N-glycans. This enzymatic activity is crucial for the modification of glycoproteins, influencing their structure and function. By specifically adding fucose in this alpha 1-6 linkage, FUT8 contributes to the diverse repertoire of glycan structures, impacting cellular recognition, signaling, and other essential biological processes. It has to highlight FUT8's specificity in glycan modification, shedding light on its role in shaping the glycoproteome and participating in various cellular functions influenced by glycosylation.
Measured by its ability to hydrolyze the donor substrate GDP fucose and the specific activity is >0.75 pmoles/min/μg.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag C-His
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Accession
Q9BYC5-1 (R68-K575)
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Molecular Construction
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N-term
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FUT8 (R68-K575)
Accession # Q9BYC5-1 -
His
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C-term
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Protein Length
Partial
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Synonyms
FUT8; Fucosyltransferase 8 (Alpha (1,6) Fucosyltransferase); Fucosyltransferase 8; Alpha1-6FucT; GDP-L-Fuc:N-Acetyl-Beta-D-Glucosaminide Alpha1,6-Fucosyltransferase; Alpha (1,6) Fucosyltransferase; GDP-Fucose--Glycoprotein Fucosyltransferase; FUT8 Protein
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AA Sequence
RIPEGPIDQGPAIGRVRVLEEQLVKAKEQIENYKKQTRNGLGKDHEILRRRIENGAKELWFFLQSELKKLKNLEGNELQRHADEFLLDLGHHERSIMTDLYYLSQTDGAGDWREKEAKDLTELVQRRITYLQNPKDCSKAKKLVCNINKGCGYGCQLHHVVYCFMIAYGTQRTLILESQNWRYATGGWETVFRPVSETCTDRSGISTGHWSGEVKDKNVQVVELPIVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKYLIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEFISDNSISWSAGLHNRYTENSLRGVILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANFHSLDDIYYFGGQNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVREKIETVKYPTYPEAEK
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Predicted Molecular Mass
60 kDa
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Molecular Weight
Approximately 55 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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