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  3. Biotin-SS-Amine hydrochloride

Biotin-SS-Amine hydrochloride is a cleavable biotinylation reagent. The amine group can react with carboxylic acids, activated NHS esters and other carbonyl groups. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.

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Biotin-SS-Amine hydrochloride

Biotin-SS-Amine hydrochloride Chemical Structure

CAS No. : 2098496-90-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
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Description

Biotin-SS-Amine hydrochloride is a cleavable biotinylation reagent. The amine group can react with carboxylic acids, activated NHS esters and other carbonyl groups. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.

Molecular Weight

415.04

Formula

C14H27ClN4O2S3

CAS No.
Appearance

Solid

SMILES

NCCSSCCNC(CCCC[C@H]1[C@]2([H])[C@](NC(N2)=O)([H])CS1)=O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (240.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4094 mL 12.0470 mL 24.0941 mL
5 mM 0.4819 mL 2.4094 mL 4.8188 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.17%

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4094 mL 12.0470 mL 24.0941 mL 60.2352 mL
5 mM 0.4819 mL 2.4094 mL 4.8188 mL 12.0470 mL
10 mM 0.2409 mL 1.2047 mL 2.4094 mL 6.0235 mL
15 mM 0.1606 mL 0.8031 mL 1.6063 mL 4.0157 mL
20 mM 0.1205 mL 0.6024 mL 1.2047 mL 3.0118 mL
25 mM 0.0964 mL 0.4819 mL 0.9638 mL 2.4094 mL
30 mM 0.0803 mL 0.4016 mL 0.8031 mL 2.0078 mL
40 mM 0.0602 mL 0.3012 mL 0.6024 mL 1.5059 mL
50 mM 0.0482 mL 0.2409 mL 0.4819 mL 1.2047 mL
60 mM 0.0402 mL 0.2008 mL 0.4016 mL 1.0039 mL
80 mM 0.0301 mL 0.1506 mL 0.3012 mL 0.7529 mL
100 mM 0.0241 mL 0.1205 mL 0.2409 mL 0.6024 mL
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Biotin-SS-Amine hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Biotin-SS-Amine hydrochloride
Cat. No.:
HY-W800651
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