Bisphenol A

Name: Bisphenol A
Brand: MedChemExpress
SKU: HY-18260
Rating: 5.0 (14 reviews)

Cat. No.: HY-18260 Purity: 99.91%: Data Sheet
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Bisphenol A is a phenolic, organic synthetic compound widely used in the production of polycarbonate plastics and epoxy resins. Bisphenol A is a reproductive, developmental, and systemic toxicant, often classified as an endocrine-disrupting compound (EDC). Bisphenol A is associated with many diseases, including cancers, cardiovascular diseases, respiratory diseases, diabetes, kidney diseases, obesity, and reproductivedisorders.

For research use only. We do not sell to patients.

CAS No. : 80-05-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of Bisphenol A:

14 Publications Citing Use of MCE Bisphenol A

In Vivo Efficacy Study

Bio/Physico-chemical Assay

Histological Imaging/Staining

: Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]; Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks) significantly downregulated GPX4 expression in the testes of mice in a dose-dependent manner.

: Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]; Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks) induced testicular atrophy and significantly decreased the index of the mice testis.

: Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]; Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks) increased iron and malondialdehyde (MDA) content while significantly decreased the GSH/GSSG ratio in mice testis.

: Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]; Histological changes and TEM imaging images of mitochondria structures in the testes of Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks)-treated mice. NST, normal seminiferous tubule; DST, damaged seminiferous tubule; LC, Leydig cells; Sg, spermatogonia. M, mitochondria; LC, loss of mitochondrial cristae; rOM, ruptured mitochondrial outer membrane; AL, autolysosome; SM, shrunken mitochondria.

: Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]; Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks) significantly upregulated the protein expression of both estrogen receptors ERα and ERβ, as well as the G protein-coupled estrogen receptor (GPER) in mice testes, in a dose-dependent manner.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	0.0086 M Compound: BAP	Compound was tested for the in vitro growth inhibition of B-16 melanoma cells after incubation for 3 days (cytotoxicity), the dose that failed to inhibit cell growth by more than 50% was determined Compound was tested for the in vitro growth inhibition of B-16 melanoma cells after incubation for 3 days (cytotoxicity), the dose that failed to inhibit cell growth by more than 50% was determined	[PMID: 9288164]
HEK293	EC₅₀	1689 nM Compound: 3; BPA	Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay	[PMID: 30940565]
HEK293	EC₅₀	> 10000 nM Compound: 3; BPA	Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay	[PMID: 30940565]
HEK293	IC₅₀	> 10000 nM Compound: 3; BPA	Antagonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as reduction in E2-induced ER-alpha-mediated transcriptional activity by luciferase reporter gene assay Antagonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as reduction in E2-induced ER-alpha-mediated transcriptional activity by luciferase reporter gene assay	[PMID: 30940565]
HT-22	IC₅₀	97 μM Compound: 33	Cytotoxicity against mouse HT-22 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against mouse HT-22 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 36876904]
HeLa	EC₅₀	757 nM Compound: BPA	Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay	[PMID: 31879182]
HeLa	EC₅₀	797 nM Compound: BPA	Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay	[PMID: 31879182]
MCF7	EC₅₀	0.29 μM Compound: 4; BPA	Agonist activity at estrogen receptor in human MCF7 cells assessed as ERE-mediated transcriptional activity after 24 hrs by luciferase assay Agonist activity at estrogen receptor in human MCF7 cells assessed as ERE-mediated transcriptional activity after 24 hrs by luciferase assay	[PMID: 26905830]

In Vitro

Bisphenol A significantly reduces the viability, increases and decreases levels of intracellular ROS production and antioxidant capacity, respectively, in KGN cells^[1].
Bisphenol A (100 fM-100 μM, 24-72 h) decreases granulosa cell viability and induces apoptosis and G2-to-M arrest of ovarian granulosa cells^[3].
Bisphenol A (0-400 μM, 24 h) suppresses proliferation and induces apoptosis in human colon cancer cells HCT116 through mitochondrial and MAPK/AKT pathways^[4].
Bisphenol A (0.5-12.5 μM, 5 days) significantly inhibits RANKL-induced, TRAP-positive multinucleated cell formation in bone marrow-derived macrophages and RAW 264.7 cell cultures^[5].
Bisphenol A (0.5-12.5 μM) suppresses ALP activities and bone nodule formation in MC3T3-E1 cells^[5].
Bisphenol A (0.06-500 μM, 1 h) induces morphological and biochemical alterations in human peripheral blood mononuclear cells^[6].
Bisphenol A (0.0020-2.0 μM, 24-48 h) decreases viability and induces dysfunction of insulin secretion and apoptosis through the damage of mitochondria in rat insulinoma (INS-1) cells^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[5]

Cell Line:	RAW264.7 cells
Concentration:	0.5, 2.5, 12.5 μM
Incubation Time:	24 h
Result:	Significantly reduced to 80% at 12.5 μM.

Apoptosis Analysis^[5]

Cell Line:	RAW264.7 cells
Concentration:	0.5, 2.5, 12.5 μM
Incubation Time:	24 h
Result:	Stimulated apoptosis in a dose-dependent manner in both cells. Up-regulated Bax and down-regulated Bcl2 in both cells.

In Vivo

Bisphenol A (200 mg/kg/day, s.c., 4 weeks) significantly decreases testis, epididymis, prostate and seminal vesicle weights, and the testicular daily sperm production in Jcl:Wistar rats^[8].
Bisphenol A (500-1250 mg/kg/day, gastric intubation, gestational days 6 through 15) induces maternal mortality and fetal toxicity in CD-1 mice^[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

228.29

Formula

C₁₅H₁₆O₂

CAS No.

80-05-7

Appearance

Solid

Color

White to off-white

SMILES

CC(C1=CC=C(O)C=C1)(C2=CC=C(O)C=C2)C

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (438.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3804 mL	21.9020 mL	43.8039 mL
5 mM	0.8761 mL	4.3804 mL	8.7608 mL
10 mM	0.4380 mL	2.1902 mL	4.3804 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (278 KB) SDS (923 KB)

COA (245 KB) RP-HPLC (258 KB)

Handling Instructions (2659 KB)

References

[1]. Huang M, et al. Bisphenol A and its analogues bisphenol S, bisphenol F and bisphenol AF induce oxidative stress and biomacromolecular damage in human granulosa KGN cells. Chemosphere. 2020 Apr 9;253:126707. [Content Brief]

[2]. Rubin BS, et al. Bisphenol A: an endocrine disruptor with widespread exposure and multiple effects. J Steroid Biochem Mol Biol. 2011 Oct;127(1-2):27-34. [Content Brief]

[3]. Xu J, et al. Bisphenol A induces apoptosis and G2-to-M arrest of ovarian granulosa cells. Biochem Biophys Res Commun. 2002 Mar 29;292(2):456-62. [Content Brief]

[4]. Qu W, et al. Bisphenol A suppresses proliferation and induces apoptosis in colonic epithelial cells through mitochondrial and MAPK/AKT pathways. Life Sci. 2018 Sep 1;208:167-174. [Content Brief]

[5]. Hwang JK, et al. Bisphenol A reduces differentiation and stimulates apoptosis of osteoclasts and osteoblasts. Life Sci. 2013 Sep 17;93(9-11):367-72. [Content Brief]

[6]. Michałowicz J, et al. Bisphenol A and its analogs induce morphological and biochemical alterations in human peripheral blood mononuclear cells (in vitro study). Toxicol In Vitro. 2015 Oct;29(7):1464-72. [Content Brief]

[7]. Lin Y, et al. Exposure to bisphenol A induces dysfunction of insulin secretion and apoptosis through the damage of mitochondria in rat insulinoma (INS-1) cells. Cell Death Dis. 2013 Jan 17;4(1):e460. [Content Brief]

[8]. Takahashi O, et al. Testicular toxicity of dietarily or parenterally administered bisphenol A in rats and mice. Food Chem Toxicol. 2003 Jul;41(7):1035-44. [Content Brief]

[9]. Morrissey RE, et al. The developmental toxicity of bisphenol A in rats and mice. Fundam Appl Toxicol. 1987 May;8(4):571-82. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3804 mL	21.9020 mL	43.8039 mL	109.5098 mL
	5 mM	0.8761 mL	4.3804 mL	8.7608 mL	21.9020 mL
	10 mM	0.4380 mL	2.1902 mL	4.3804 mL	10.9510 mL
	15 mM	0.2920 mL	1.4601 mL	2.9203 mL	7.3007 mL
	20 mM	0.2190 mL	1.0951 mL	2.1902 mL	5.4755 mL
	25 mM	0.1752 mL	0.8761 mL	1.7522 mL	4.3804 mL
	30 mM	0.1460 mL	0.7301 mL	1.4601 mL	3.6503 mL
	40 mM	0.1095 mL	0.5475 mL	1.0951 mL	2.7377 mL
	50 mM	0.0876 mL	0.4380 mL	0.8761 mL	2.1902 mL
	60 mM	0.0730 mL	0.3650 mL	0.7301 mL	1.8252 mL
	80 mM	0.0548 mL	0.2738 mL	0.5475 mL	1.3689 mL
	100 mM	0.0438 mL	0.2190 mL	0.4380 mL	1.0951 mL

Bisphenol A Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bisphenol A80-05-7Aktp38 MAPKReactive Oxygen Species (ROS)Environmental PollutantsEndogenous MetaboliteApoptosisPKBProtein kinase BEnvironmental Contaminantsendocrine-disruptingcompoundEDCphenolictoxicantInhibitorinhibitorinhibit

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Bisphenol A

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Other Forms of Bisphenol A:

Bisphenol A Related Antibodies

14 Publications Citing Use of MCE Bisphenol A

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Bisphenol A Related Antibodies

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Complete Stock Solution Preparation Table

Bisphenol A Related Classifications

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