1. Apoptosis TGF-beta/Smad Epigenetics Autophagy
  2. Apoptosis PKC Autophagy
  3. C8-Ceramide

C8-Ceramide  (Synonyms: N-Octanoyl-D-erythro-sphingosine)

Cat. No.: HY-108391 Purity: ≥98.0%
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C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) is a cell-permeable analog of naturally occurring ceramides. C8-Ceramide has anti-proliferation properties and acts as a potent chemotherapeutic agent. C8-Ceramide stimulates dendritic cells to promote T cell responses upon virus infections. C8-Ceramide induces slight activation of protein kinase (PKC) in vitro.

For research use only. We do not sell to patients.

C8-Ceramide Chemical Structure

C8-Ceramide Chemical Structure

CAS No. : 74713-59-0

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10 mg USD 120 In-stock
25 mg USD 250 In-stock
50 mg USD 450 In-stock
100 mg USD 750 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

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Description

C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) is a cell-permeable analog of naturally occurring ceramides. C8-Ceramide has anti-proliferation properties and acts as a potent chemotherapeutic agent. C8-Ceramide stimulates dendritic cells to promote T cell responses upon virus infections. C8-Ceramide induces slight activation of protein kinase (PKC) in vitro[1][2][3][4].

IC50 & Target[2][4][5]

PKC

 

apoptosis

 

autophagy

 

In Vitro

C8-ceramide (3 μM; 48 hours) irreversibly reduces tumor-cell proliferation and induces morphological changes[1].
C8-ceramide can induce necrosis-like cell death, but does not induce caspase-dependent cleavage of PARP (biochemical marker of apoptosis) in human cervical tumor cells[1].
C8-ceramide may increase the endogenous ROS level (10-30 µM; 24 hours) by regulating the switch of SOD1 and SOD2, causing the anti-proliferation (10-50 µM; 24 hours), and consequently triggering the apoptosis (10-50 µM; 48 hours) of NSCLC H1299 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: CALO cells, INBL cells, HeLa cells
Concentration: 3 μM
Incubation Time: 48 hours
Result: Markedly reduced the tumor cell number.

Cell Proliferation Assay[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time: 24 hours
Result: Decreased the rate of cellular proliferation in a dose-dependent manner, with an IC50 of 22.9 µM.

Cell Cycle Analysis[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time: 24 hours
Result: Caused the G1 arrest.

Apoptosis Analysis[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM
Incubation Time: 24 hours, 48 hours
Result: Increased the level of cleaved caspase-3.
In Vivo

C8-ceramide (0.1 mg/kg; intranasal administration) induces more robust CD8+ and CD4+ T cell responses to viral infections in virus infected mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice, with lymphocytic choriomeningitis virus infected[3]
Dosage: 0.1 mg/kg
Administration: Intranasal administration
Result: Increased the CD8+ T cell response to influenza in the lungs.
Molecular Weight

425.69

Formula

C26H51NO3

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCC(N[C@@H](CO)[C@H](O)/C=C/CCCCCCCCCCCCC)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (234.91 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3491 mL 11.7456 mL 23.4913 mL
5 mM 0.4698 mL 2.3491 mL 4.6983 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3491 mL 11.7456 mL 23.4913 mL 58.7282 mL
5 mM 0.4698 mL 2.3491 mL 4.6983 mL 11.7456 mL
10 mM 0.2349 mL 1.1746 mL 2.3491 mL 5.8728 mL
15 mM 0.1566 mL 0.7830 mL 1.5661 mL 3.9152 mL
20 mM 0.1175 mL 0.5873 mL 1.1746 mL 2.9364 mL
25 mM 0.0940 mL 0.4698 mL 0.9397 mL 2.3491 mL
30 mM 0.0783 mL 0.3915 mL 0.7830 mL 1.9576 mL
40 mM 0.0587 mL 0.2936 mL 0.5873 mL 1.4682 mL
50 mM 0.0470 mL 0.2349 mL 0.4698 mL 1.1746 mL
60 mM 0.0392 mL 0.1958 mL 0.3915 mL 0.9788 mL
80 mM 0.0294 mL 0.1468 mL 0.2936 mL 0.7341 mL
100 mM 0.0235 mL 0.1175 mL 0.2349 mL 0.5873 mL
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C8-Ceramide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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C8-Ceramide
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HY-108391
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