1. Apoptosis
    TGF-beta/Smad
    Epigenetics
    Autophagy
  2. Apoptosis
    PKC
    Autophagy
  3. C8-Ceramide

C8-Ceramide (Synonyms: N-Octanoyl-D-erythro-sphingosine)

Cat. No.: HY-108391 Purity: ≥98.0%
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C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) is a cell-permeable analog of naturally occurring ceramides. C8-Ceramide has anti-proliferation properties and acts as a potent chemotherapeutic agent. C8-Ceramide stimulates dendritic cells to promote T cell responses upon virus infections. C8-Ceramide induces slight activation of protein kinase (PKC) in vitro.

For research use only. We do not sell to patients.

C8-Ceramide Chemical Structure

C8-Ceramide Chemical Structure

CAS No. : 74713-59-0

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Description

C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) is a cell-permeable analog of naturally occurring ceramides. C8-Ceramide has anti-proliferation properties and acts as a potent chemotherapeutic agent. C8-Ceramide stimulates dendritic cells to promote T cell responses upon virus infections. C8-Ceramide induces slight activation of protein kinase (PKC) in vitro[1][2][3][4].

IC50 & Target[2][4][5]

PKC

 

apoptosis

 

autophagy

 

In Vitro

C8-ceramide (3 μM; 48 hours) irreversibly reduces tumor-cell proliferation and induces morphological changes[1].
C8-ceramide can induce necrosis-like cell death, but does not induce caspase-dependent cleavage of PARP (biochemical marker of apoptosis) in human cervical tumor cells[1].
C8-ceramide may increase the endogenous ROS level (10-30 µM; 24 hours) by regulating the switch of SOD1 and SOD2, causing the anti-proliferation (10-50 µM; 24 hours), and consequently triggering the apoptosis (10-50 µM; 48 hours) of NSCLC H1299 cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: CALO cells, INBL cells, HeLa cells
Concentration: 3 μM
Incubation Time: 48 hours
Result: Markedly reduced the tumor cell number.

Cell Proliferation Assay[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time: 24 hours
Result: Decreased the rate of cellular proliferation in a dose-dependent manner, with an IC50 of 22.9 µM.

Cell Cycle Analysis[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time: 24 hours
Result: Caused the G1 arrest.

Apoptosis Analysis[2]

Cell Line: H1299 cells
Concentration: 10 µM, 20 µM, 30 µM
Incubation Time: 24 hours, 48 hours
Result: Increased the level of cleaved caspase-3.
In Vivo

C8-ceramide (0.1 mg/kg; intranasal administration) induces more robust CD8+ and CD4+ T cell responses to viral infections in virus infected mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice, with lymphocytic choriomeningitis virus infected[3]
Dosage: 0.1 mg/kg
Administration: Intranasal administration
Result: Increased the CD8+ T cell response to influenza in the lungs.
Molecular Weight

425.69

Formula

C₂₆H₅₁NO₃

CAS No.
SMILES

CCCCCCCC(N[[email protected]@H](CO)[[email protected]](O)/C=C/CCCCCCCCCCCCC)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

References

Purity: ≥98.0%

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Keywords:

C8-CeramideN-Octanoyl-D-erythro-sphingosineApoptosisPKCAutophagyProtein kinase Cceramidesanaloganti-proliferationcytotoxicchemotherapeuticagent,proteinkinaseactivationInhibitorinhibitorinhibit

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C8-Ceramide
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