Carboxy SNARF-1, acetoxymethyl ester (5/6-mixture)

Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).

2. Working Solution Preparation
2.2 Working concentration: 5 μM.
2.3 Note: Adjust working solution concentration as needed; prepare fresh before use.

3. Staining Procedure
3.1 Sample type: Isolated rat portal vein smooth muscle tissue strips.[1]
3.2 Incubation conditions: Incubate tissue strips with 5 μM carboxy SNARF, acetoxymethyl ester for 1 h at room temperature.

5. Detection & Analysis
5.1 Instrument: Inverted fluorescence microscope.
5.1.1 Excitation wavelength: 540 nm; emission wavelengths: 590 nm and 650 nm.
5.2 Result analysis:
5.2.1 Calculate the ratio of emission signals at 590 nm and 650 nm to obtain linear intracellular pH measurements.
5.2.2 Calibrate pH measurements using either nigericin or an in vitro mock intracellular solution with known pH values.
5.2.3 Stable fluorescence recordings for intracellular pH measurement can be obtained for 1-2 h following loading.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.