Carboxylated magnetic microspheres (low non-specificity)

Carboxylated magnetic microspheres (low nonspecificity) are functionalized nano- or micron-sized magnetic particles with abundant carboxyl functional groups on their surface. Special chemical treatments reduce the likelihood of nonspecific adsorption. This 1μm carboxylated magnetic microsphere features a core-shell structure: a PS core, an outer layer of iron oxide (Fe₃O₄), and an outermost encapsulation layer. The carboxyl groups are obtained through polymer modification. Exhibiting low nonspecific adsorption, these microspheres can covalently couple with bioligands such as peptides, proteins, antibodies, and oligonucleotides using specific chemical reagents (e.g., EDC), making them particularly suitable for cell sorting, affinity chromatography, and immunoassay.