Ethyl 2-cyano-2-(hydroxyimino)acetate
Based on 1 Customer Validation
Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma) is a peptide coupling additive. Ethyl 2-cyano-2-(hydroxyimino) acetate suppresses racemization during peptide coupling and improves the coupling efficiency of both solution-phase and solid-phase peptide synthesis.
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- Pureté: 99.89%
- CAS No.: 3849-21-6
- Formule: C5H6N2O3
- Masse moléculaire:142.11
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Chemical Information
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CAS No. 3849-21-6
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Appearance Solid
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Masse moléculaire 142.11
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Formule C5H6N2O3
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Color White to light yellow
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SMILES
O=C(OCC)/C(C#N)=N/O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 100 mg/mL (703.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (270 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 7.0368 mL | 35.1840 mL | 70.3680 mL | 175.9201 mL |
| 5 mM | 1.4074 mL | 7.0368 mL | 14.0736 mL | 35.1840 mL | |
| 10 mM | 0.7037 mL | 3.5184 mL | 7.0368 mL | 17.5920 mL | |
| 15 mM | 0.4691 mL | 2.3456 mL | 4.6912 mL | 11.7280 mL | |
| 20 mM | 0.3518 mL | 1.7592 mL | 3.5184 mL | 8.7960 mL | |
| 25 mM | 0.2815 mL | 1.4074 mL | 2.8147 mL | 7.0368 mL | |
| 30 mM | 0.2346 mL | 1.1728 mL | 2.3456 mL | 5.8640 mL | |
| 40 mM | 0.1759 mL | 0.8796 mL | 1.7592 mL | 4.3980 mL | |
| 50 mM | 0.1407 mL | 0.7037 mL | 1.4074 mL | 3.5184 mL | |
| 60 mM | 0.1173 mL | 0.5864 mL | 1.1728 mL | 2.9320 mL | |
| 80 mM | 0.0880 mL | 0.4398 mL | 0.8796 mL | 2.1990 mL | |
| 100 mM | 0.0704 mL | 0.3518 mL | 0.7037 mL | 1.7592 mL |
Ethyl 2-cyano-2-(hydroxyimino)acetate Related Classifications
HY-41456 Related Classifications
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- Ethyl 2-cyano-2-(hydroxyimino)acetate
- 3849-21-6
- Biochemical Assay Reagents
- nitriles
- racemization
- aliphatic aldoximes
- aromatic aldoximes
- peptide coupling additive
- Leu-enkephalin
- solid-phase peptide synthesis
- aldoxime dehydration promoter
- solution-phase peptide synthesis
- benzotriazole-based additives
- Inhibitor
- inhibitor
- inhibit