Cat. No.: HY-P0288

[Leu5]-Enkephalin

(Synonyms: Leu-enkephalin; Leucine enkephalin; Leucyl-enkephalin)

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

[Leu5]-Enkephalin is a pentapeptide with morphine like properties. [Leu5]-Enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

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Pureté: 99.92%
CAS No.: 58822-25-6
Formule: C₂₈H₃₇N₅O₇
Masse moléculaire:555.62

Stockage:

Sealed storage, away from moisture.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

[Leu5]-Enkephalin Chemical Structure

CAS No.: 58822-25-6

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

[Leu5]-Enkephalin is a pentapeptide with morphine like properties. [Leu5]-Enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

IC₅₀ & Target

Human Endogenous Metabolite

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Voir tous les produits spécifiques à Isoform Endogenous Metabolite :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	0.08 nM Compound: 105	Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells	[PMID: 31377035]
CHO	EC₅₀	1.3 nM Compound: 105	Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells	[PMID: 31377035]
CHO	EC₅₀	80 nM Compound: 105	Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells	[PMID: 31377035]
CHO	EC₅₀	80 nM Compound: 283	Activation of kappa opioid receptor (unknown origin) expressed in CHO cells Activation of kappa opioid receptor (unknown origin) expressed in CHO cells	[PMID: 29400967]

In Vitro

Enkephalins (met- , leu-enkephalin, and enkephalin 8) and dynorphins are two classes of opioid peptides found in the spinal dorsal horn. Mu, delta, and kappa are three major subtypes of opioid receptors. Enkephalins are putative endogenous ligands for delta opioid receptors, and dynorphins are endogenous ligands for the kappa opioid receptors. Three receptor types resembling the vertebrate δ- and κ-type opioid receptors have been characterized pharmacologically in nervous tissues (e.g. K_i=18.9 nM for Leu-enkephalin) and localized by autoradiography at CHH terminals in the SG of C. maenas^[1].
Leucine-enkephalin is a pentapeptides with morphine like properties, naturally present in mammalian brain^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 58822-25-6
Appearance Solid
Masse moléculaire 555.62
Formule C₂₈H₃₇N₅O₇
Color White to light yellow
Synonyms

Leu-enkephalin; Leucine enkephalin; Leucyl-enkephalin
Sequence

Tyr-Gly-Gly-Phe-Leu
Sequence Shortening

YGGFL
Structure Classification
- Phenols
- Monophenols
- Others
Initial Source
- Endogenous metabolite
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (2)

All Cell Proliferation/Viability AssayCell Imaging/StainingWB

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Journal Impact Factor

ACS Omega

ZnO Nanocages Decorated with Au@AgAu Yolk-Shell Nanomaterials for SERS-Based Detection of Hyperuricemia. [Abstract]

2024 Mar 28;9(14):16160-16167. PMID: 38617613
World J Acupunct Moxibustion

2025 Jun 3.

[Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

[Leu5]-Enkephalin (1-200 μM; 24 h) did not exhibit cytotoxicity in ARPE-19 cells (P > 0.05).

[Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

[Leu5]-Enkephalin (10-30 µM; 24 h) significantly ameliorated the OGD-induced reduction in ARPE-19 cell viability.

[Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

Impacts of naltrindole and enkephalins treatment on cell viability, morphological changes, and apoptosis rates in OGD/R-injured ARPE-19 cells (mean ± SEM, n = 4 or 5 replicates per group, scale bar = 200 µm). The results showed that following treatment with [Leu5]-Enkephalin (MEK), the cell count increased significantly, and cell morphology reverted to a normal state.

[Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

Effects of naloxone and enkephalin on DOR expression in total cellular and membrane protein fractions of ARPE-19 cells under OGD/R (mean ± SEM, n = 4 independent experiments). The results showed that treatment with [Leu5]-Enkephalin (MEK) increased DOR levels in both total proteins (P <0.05) and membrane proteins (P < 0.001).

Solvant et solubilité

In Vitro:

DMSO : 116.67 mg/mL (209.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (179.98 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7998 mL

8.9990 mL

17.9979 mL

5 mM

0.3600 mL

1.7998 mL

3.5996 mL

10 mM

0.1800 mL

0.8999 mL

1.7998 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 6.67 mg/mL (12.00 mM); Clear solution; Need ultrasonic

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (272 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Leu-enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

[2]. Meunier JC, et al. Binding of Leu5-enkephalin and Met5-enkephalin to a particulate fraction from rat cerebrum. FEBS Lett. 1977 May 15;77(2):209-13. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	1.7998 mL	8.9990 mL	17.9979 mL	44.9948 mL
	5 mM	0.3600 mL	1.7998 mL	3.5996 mL	8.9990 mL
	10 mM	0.1800 mL	0.8999 mL	1.7998 mL	4.4995 mL
	15 mM	0.1200 mL	0.5999 mL	1.1999 mL	2.9997 mL
	20 mM	0.0900 mL	0.4499 mL	0.8999 mL	2.2497 mL
	25 mM	0.0720 mL	0.3600 mL	0.7199 mL	1.7998 mL
	30 mM	0.0600 mL	0.3000 mL	0.5999 mL	1.4998 mL
	40 mM	0.0450 mL	0.2250 mL	0.4499 mL	1.1249 mL
	50 mM	0.0360 mL	0.1800 mL	0.3600 mL	0.8999 mL
	60 mM	0.0300 mL	0.1500 mL	0.3000 mL	0.7499 mL
	80 mM	0.0225 mL	0.1125 mL	0.2250 mL	0.5624 mL
	100 mM	0.0180 mL	0.0900 mL	0.1800 mL	0.4499 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

[Leu5]-Enkephalin

2 Cited Publications

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[Leu5]-Enkephalin

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Publications Citing Use of MedChemExpress (MCE) [Leu5]-Enkephalin

Voir tous les produits spécifiques à Isoform Opioid Receptor

Voir tous les produits spécifiques à Isoform Endogenous Metabolite

Activité biologique

Chemical Information

Publications (2)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

[Leu5]-Enkephalin Related Classifications

HY-P0288 Related Classifications