[Leu5]-Enkephalin
Based on 2 publication(s) in Google Scholar
[Leu5]-Enkephalin is a pentapeptide with morphine like properties. [Leu5]-Enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.
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- Pureté: 99.92%
- CAS No.: 58822-25-6
- Formule: C28H37N5O7
- Masse moléculaire:555.62
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Stockage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications Citing Use of MedChemExpress (MCE) [Leu5]-Enkephalin
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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WB
Voir tous les produits spécifiques à Isoform Opioid Receptor
MoreVoir tous les produits spécifiques à Isoform Endogenous Metabolite
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Activité biologique
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | EC50 |
0.08 nM
Compound: 105
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Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells
Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells
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[PMID: 31377035] |
| CHO | EC50 |
1.3 nM
Compound: 105
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Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells
Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells
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[PMID: 31377035] |
| CHO | EC50 |
80 nM
Compound: 105
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Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells
Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells
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[PMID: 31377035] |
| CHO | EC50 |
80 nM
Compound: 283
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Activation of kappa opioid receptor (unknown origin) expressed in CHO cells
Activation of kappa opioid receptor (unknown origin) expressed in CHO cells
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[PMID: 29400967] |
Enkephalins (met- , leu-enkephalin, and enkephalin 8) and dynorphins are two classes of opioid peptides found in the spinal dorsal horn. Mu, delta, and kappa are three major subtypes of opioid receptors. Enkephalins are putative endogenous ligands for delta opioid receptors, and dynorphins are endogenous ligands for the kappa opioid receptors. Three receptor types resembling the vertebrate δ- and κ-type opioid receptors have been characterized pharmacologically in nervous tissues (e.g. Ki=18.9 nM for Leu-enkephalin) and localized by autoradiography at CHH terminals in the SG of C. maenas[1].
Leucine-enkephalin is a pentapeptides with morphine like properties, naturally present in mammalian brain[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 58822-25-6
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Appearance Solid
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Masse moléculaire 555.62
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Formule C28H37N5O7
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Color White to light yellow
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Synonyms
Leu-enkephalin; Leucine enkephalin; Leucyl-enkephalin
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Sequence
Tyr-Gly-Gly-Phe-Leu
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Sequence Shortening
YGGFL
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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ACS Omega
ZnO Nanocages Decorated with Au@AgAu Yolk-Shell Nanomaterials for SERS-Based Detection of Hyperuricemia. [Abstract]2024 Mar 28;9(14):16160-16167. PMID: 38617613 -
[Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.
[Leu5]-Enkephalin (1-200 μM; 24 h) did not exhibit cytotoxicity in ARPE-19 cells (P > 0.05).
[Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.
[Leu5]-Enkephalin (10-30 µM; 24 h) significantly ameliorated the OGD-induced reduction in ARPE-19 cell viability.
[Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.
Impacts of naltrindole and enkephalins treatment on cell viability, morphological changes, and apoptosis rates in OGD/R-injured ARPE-19 cells (mean ± SEM, n = 4 or 5 replicates per group, scale bar = 200 µm). The results showed that following treatment with [Leu5]-Enkephalin (MEK), the cell count increased significantly, and cell morphology reverted to a normal state.
[Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.
Effects of naloxone and enkephalin on DOR expression in total cellular and membrane protein fractions of ARPE-19 cells under OGD/R (mean ± SEM, n = 4 independent experiments). The results showed that treatment with [Leu5]-Enkephalin (MEK) increased DOR levels in both total proteins (P <0.05) and membrane proteins (P < 0.001).
Solvant et solubilité
DMSO : 116.67 mg/mL (209.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (179.98 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 6.67 mg/mL (12.00 mM); Clear solution; Need ultrasonic
Pureté et documentation
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Fiche technique (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.7998 mL | 8.9990 mL | 17.9979 mL | 44.9948 mL |
| 5 mM | 0.3600 mL | 1.7998 mL | 3.5996 mL | 8.9990 mL | |
| 10 mM | 0.1800 mL | 0.8999 mL | 1.7998 mL | 4.4995 mL | |
| 15 mM | 0.1200 mL | 0.5999 mL | 1.1999 mL | 2.9997 mL | |
| 20 mM | 0.0900 mL | 0.4499 mL | 0.8999 mL | 2.2497 mL | |
| 25 mM | 0.0720 mL | 0.3600 mL | 0.7199 mL | 1.7998 mL | |
| 30 mM | 0.0600 mL | 0.3000 mL | 0.5999 mL | 1.4998 mL | |
| 40 mM | 0.0450 mL | 0.2250 mL | 0.4499 mL | 1.1249 mL | |
| 50 mM | 0.0360 mL | 0.1800 mL | 0.3600 mL | 0.8999 mL | |
| 60 mM | 0.0300 mL | 0.1500 mL | 0.3000 mL | 0.7499 mL | |
| 80 mM | 0.0225 mL | 0.1125 mL | 0.2250 mL | 0.5624 mL | |
| 100 mM | 0.0180 mL | 0.0900 mL | 0.1800 mL | 0.4499 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.