2. GPCR/G Protein Neuronal Signaling Metabolic Enzyme/Protease
  3. Opioid Receptor Endogenous Metabolite
  4. [Leu5]-Enkephalin

[Leu5]-Enkephalin  (Synonyms: Leu-enkephalin; Leucine enkephalin; Leucyl-enkephalin)

Cat. No.: HY-P0288 Purity: 99.92%
COA
SDS
Handling Instructions Technical Support

[Leu5]-Enkephalin is a pentapeptide with morphine like properties. [Leu5]-Enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 58822-25-6

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of [Leu5]-Enkephalin:

[Leu5]-Enkephalin Related Antibodies

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
Cell Imaging/Staining
WB

    [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

    [Leu5]-Enkephalin (1-200 μM; 24 h) did not exhibit cytotoxicity in ARPE-19 cells (P > 0.05).

    [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

    [Leu5]-Enkephalin (10-30 µM; 24 h) significantly ameliorated the OGD-induced reduction in ARPE-19 cell viability.

    [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

    Impacts of naltrindole and enkephalins treatment on cell viability, morphological changes, and apoptosis rates in OGD/R-injured ARPE-19 cells (mean ± SEM, n = 4 or 5 replicates per group, scale bar = 200 µm). The results showed that following treatment with [Leu5]-Enkephalin (MEK), the cell count increased significantly, and cell morphology reverted to a normal state.

    [Leu5]-Enkephalin purchased from MedChemExpress. Usage Cited in: World J Acupunct Moxibustion. 2025 Jun 3.

    Effects of naloxone and enkephalin on DOR expression in total cellular and membrane protein fractions of ARPE-19 cells under OGD/R (mean ± SEM, n = 4 independent experiments). The results showed that treatment with [Leu5]-Enkephalin (MEK) increased DOR levels in both total proteins (P <0.05) and membrane proteins (P < 0.001).

    View All Opioid Receptor Isoform Specific Products:

    View All Isoforms
    μ Opioid Receptor/MOR κ Opioid Receptor/KOR δ Opioid Receptor/DOR NOP Receptor/ORL1

    View All Endogenous Metabolite Isoform Specific Products:

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    [Leu5]-Enkephalin is a pentapeptide with morphine like properties. [Leu5]-Enkephalin is a five amino acid endogenous peptide that acts as an agonist at opioid receptors.

    IC50 & Target

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    CHO EC50
    0.08 nM
    Compound: 105
    Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells
    Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells
    		[PMID: 31377035]
    CHO EC50
    1.3 nM
    Compound: 105
    Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells
    Agonist activity at mu opioid receptor (unknown origin) expressed in CHO cells
    		[PMID: 31377035]
    CHO EC50
    80 nM
    Compound: 105
    Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells
    Agonist activity at kappa opioid receptor (unknown origin) expressed in CHO cells
    		[PMID: 31377035]
    CHO EC50
    80 nM
    Compound: 283
    Activation of kappa opioid receptor (unknown origin) expressed in CHO cells
    Activation of kappa opioid receptor (unknown origin) expressed in CHO cells
    		[PMID: 29400967]
    In Vitro

    Enkephalins (met- , leu-enkephalin, and enkephalin 8) and dynorphins are two classes of opioid peptides found in the spinal dorsal horn. Mu, delta, and kappa are three major subtypes of opioid receptors. Enkephalins are putative endogenous ligands for delta opioid receptors, and dynorphins are endogenous ligands for the kappa opioid receptors. Three receptor types resembling the vertebrate δ- and κ-type opioid receptors have been characterized pharmacologically in nervous tissues (e.g. Ki=18.9 nM for Leu-enkephalin) and localized by autoradiography at CHH terminals in the SG of C. maenas[1].
    Leucine-enkephalin is a pentapeptides with morphine like properties, naturally present in mammalian brain[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    [Leu5]-Enkephalin Related Antibodies
    Molecular Weight

    555.62

    Formula

    C28H37N5O7
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    Sequence

    Tyr-Gly-Gly-Phe-Leu
    Sequence Shortening

    YGGFL
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 116.67 mg/mL (209.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (179.98 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7998 mL 8.9990 mL 17.9979 mL
    5 mM 0.3600 mL 1.7998 mL 3.5996 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 6.67 mg/mL (12.00 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.92%

    SDS (252 KB)

    COA (245 KB) RP-HPLC (251 KB) MS (68 KB) Elemental Analysis Report (104 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.7998 mL 8.9990 mL 17.9979 mL 44.9948 mL
    5 mM 0.3600 mL 1.7998 mL 3.5996 mL 8.9990 mL
    10 mM 0.1800 mL 0.8999 mL 1.7998 mL 4.4995 mL
    15 mM 0.1200 mL 0.5999 mL 1.1999 mL 2.9997 mL
    20 mM 0.0900 mL 0.4499 mL 0.8999 mL 2.2497 mL
    25 mM 0.0720 mL 0.3600 mL 0.7199 mL 1.7998 mL
    30 mM 0.0600 mL 0.3000 mL 0.5999 mL 1.4998 mL
    40 mM 0.0450 mL 0.2250 mL 0.4499 mL 1.1249 mL
    50 mM 0.0360 mL 0.1800 mL 0.3600 mL 0.8999 mL
    60 mM 0.0300 mL 0.1500 mL 0.3000 mL 0.7499 mL
    80 mM 0.0225 mL 0.1125 mL 0.2250 mL 0.5624 mL
    100 mM 0.0180 mL 0.0900 mL 0.1800 mL 0.4499 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    [Leu5]-Enkephalin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    [Leu5]-Enkephalin58822-25-6Leu-enkephalin Leucine enkephalin Leucyl-enkephalinOpioid ReceptorEndogenous MetaboliteInhibitorinhibitorinhibit

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