Cat. No.: HY-N9447

Norbergenin

1 Cited Publications

Avis client

Based on 1 publication(s) in Google Scholar

Norbergenin is a polyphenolic isocoumarin derivative. Norbergenin acts as a non-competitive inhibitor of bovine adrenal tyrosine hydroxylase (TH) with a K_i value of 69.6 μM. Norbergenin inhibits lipopolysaccharide-induced inflammatory responses in macrophages by suppressing the activation of NF-κB, MAPK and STAT3, as well as blocking metabolic reprogramming. Norbergenin inhibits aluminum chloride-induced oxidative stress and apoptosis and restores neurocognitive parameters. Norbergenin scavenges ROS through multiple mechanisms and protects cell membranes from lipid peroxidation damage. Norbergenin can be used in research related to Alzheimer's disease, peptic ulcer and arthritis.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Pureté: 98.31%
CAS No.: 79595-97-4
Formule: C₁₃H₁₄O₉
Masse moléculaire:314.24

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Norbergenin Chemical Structure

CAS No.: 79595-97-4

Size

Prix

Stock

Quantité

Amount: USD 0.00

* Veuillez sélectionner la quantité avant d'ajouter des articles.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Voir tous les produits spécifiques à Isoform NF-κB :

Voir tous les produits spécifiques à Isoform p38 MAPK :

Voir tous les produits spécifiques à Isoform STAT :

In Vitro

Norbergenin (5-50 μM; 25 h) dose-dependently inhibits LPS (HY-D1056)-induced NO production in iBMDMs at 24 h post-stimulation^[2].
Norbergenin (5-50 μM; 25 h) dose-dependently inhibits LPS-induced TNFα production in iBMDMs at 24 h post-stimulation^[2].
Norbergenin (50 μM; 2-25 h) inhibits LPS-induced mRNA expression of *Il1b*, *Tnfa*, *Il6* and *Nos2* in iBMDM and BMDM^[2].
Norbergenin (5-50 μM; 5-9 h) dose-dependently inhibits LPS-induced activation of NF-κB, STAT3 and MAPK (p38, JNK, ERK) signaling pathways, and reduces the protein levels of pro-IL1β, iNOS and TLR2 in stimulated iBMDMs at 4 h or 8 h^[2].
Norbergenin (5-50 μM; 9 h) dose-dependently inhibits LPS-induced glycolysis and maintains mitochondrial oxidative phosphorylation (OXPHOS) in iBMDMs at 8 h post-stimulation^[2].
Norbergenin (50 μM; 9 h) alters the proteome profile of iBMDMs stimulated with LPS for 8 h, downregulates proteins related to inflammatory pathways and upregulates proteins related to oxidative phosphorylation, thereby restoring its profile to a state similar to that of the unstimulated control group^[2].
Norbergenin (50 μM; 9 h) reverses LPS-induced metabolic reprogramming in iBMDMs after 8 hours, restoring normal activity of glycolysis, tricarboxylic acid (TCA) cycle, and arginine metabolism pathways^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

murine immortalized bone marrow-derived macrophages (iBMDMs), primary bone marrow-derived macrophages (BMDMs)
Concentration:

1, 5, 10 and 50 μM
Incubation Time:

24 h
Result:

Showed no significant toxic effects on iBMDMs and BMDMs at concentrations between 1 to 50 μM.

ELISA Assay^[2]

Cell Line:

murine immortalized bone marrow-derived macrophages (iBMDMs)
Concentration:

5, 10 and 50 μM
Incubation Time:

1 h preincubation; 24 h LPS stimulation
Result:

Significantly reduced LPS-induced TNFα production in a dose-dependent manner, with the 50 μM concentration showing the greatest inhibitory effect.

Real Time qPCR^[2]

Cell Line:

murine immortalized bone marrow-derived macrophages (iBMDMs), primary bone marrow-derived macrophages (BMDMs)
Concentration:

50 μM
Incubation Time:

1 h preincubation; 1-24 h LPS stimulation
Result:

Significantly reduced LPS-induced mRNA expression of Il1b, Tnfa, Il6, and Nos2 at multiple time points (8, 16, 24 h) in both iBMDMs and BMDMs, with statistically significant decreases indicated by *p < 0.05**, **p < 0.01**, and ***p < 0.001.

Western Blot Analysis^[2]

Cell Line:

murine immortalized bone marrow-derived macrophages (iBMDMs)
Concentration:

5, 10 and 50 μM
Incubation Time:

1 h preincubation; 4-8 h LPS stimulation
Result:

Caused a dose-dependent reduction in LPS-induced pro-IL1β, iNOS, p-NF-κB, p-IκB-α, p-STAT3, p-p38, p-JNK, p-ERK1/2, and TLR2 protein levels.
Had no effect on LPS-induced TLR4 expression.

In Vivo

Norbergenin (5-10 mg/kg; once daily; 7 days (prophylactic model), 15 days (curative model)) dose-dependently attenuates aluminium chloride-induced Alzheimer's disease-like pathology in male Wistar rats via suppression of oxidative stress and apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 79595-97-4
Appearance Solid
Masse moléculaire 314.24
Formule C₁₃H₁₄O₉
Color White to yellow
SMILES

OC1=C2[C@@]3([H])[C@@](OC(C2=CC(O)=C1O)=O)([H])[C@H]([C@@H]([C@H](O3)CO)O)O
Structure Classification
- Phenols
- Polyphenols
- Others
Initial Source
- Plants
- other families
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

All

Sort by:

Journal Impact Factor

Chemistry Africa

2023 Apr 20.

Solvant et solubilité

In Vitro:

DMSO : 125 mg/mL (397.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1823 mL

15.9114 mL

31.8228 mL

5 mM

0.6365 mL

3.1823 mL

6.3646 mL

10 mM

0.3182 mL

1.5911 mL

3.1823 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (6.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (287 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Nalla S, et al. Kaempferide and Norbergenin avert aluminium chloride-induced amyloid β accumulation and neurocognitive shutdown via oxidative and apoptotic mechanisms. International journal of immunopathology and pharmacology. 2025;39:3946320251343687. [Content Brief]

[2]. Li W, et al. Norbergenin prevents LPS-induced inflammatory responses in macrophages through inhibiting NFκB, MAPK and STAT3 activation and blocking metabolic reprogramming. Frontiers in immunology. 2023;14:1117638. [Content Brief]

[3]. Zhang YH, et al. In vitro inhibitory effects of bergenin and norbergenin on bovine adrenal tyrosine hydroxylase. Phytotherapy research : PTR. 2003 Sep;17(8):967-9. [Content Brief]

[4]. Haq KU, et al. Elucidation of reactive oxygen species scavenging pathways of norbergenin utilizing DFT approaches. R Soc Open Sci. 2022 Dec 21;9(12):221349.
[Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1823 mL	15.9114 mL	31.8228 mL	79.5570 mL
	5 mM	0.6365 mL	3.1823 mL	6.3646 mL	15.9114 mL
	10 mM	0.3182 mL	1.5911 mL	3.1823 mL	7.9557 mL
	15 mM	0.2122 mL	1.0608 mL	2.1215 mL	5.3038 mL
	20 mM	0.1591 mL	0.7956 mL	1.5911 mL	3.9779 mL
	25 mM	0.1273 mL	0.6365 mL	1.2729 mL	3.1823 mL
	30 mM	0.1061 mL	0.5304 mL	1.0608 mL	2.6519 mL
	40 mM	0.0796 mL	0.3978 mL	0.7956 mL	1.9889 mL
	50 mM	0.0636 mL	0.3182 mL	0.6365 mL	1.5911 mL
	60 mM	0.0530 mL	0.2652 mL	0.5304 mL	1.3260 mL
	80 mM	0.0398 mL	0.1989 mL	0.3978 mL	0.9945 mL
	100 mM	0.0318 mL	0.1591 mL	0.3182 mL	0.7956 mL

Norbergenin

1 Cited Publications

100 mg Obtenir un devis

Subscribe to the

MedChemExpress

Newsletter

Nom du produit:
Cat. No.:
Quantité:
MCE Japan Authorized Agent:

Nom du produit:
CAS No.:
Species:
Pureté:
Quantité:

Norbergenin

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Heterocyclic Compound Library

Radioprotector Library

Bioactive Compound Library Max

Natural Product Library Plus

Bioactive Compound Library

Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Phenols Library

Glycolysis Compound Library

Pyroptosis Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Alzheimer's Disease Compound Library

Publications Citing Use of MedChemExpress (MCE) Norbergenin

Voir tous les produits spécifiques à Isoform NF-κB

Voir tous les produits spécifiques à Isoform p38 MAPK

Voir tous les produits spécifiques à Isoform STAT

Activité biologique

Chemical Information

Publications (1)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Norbergenin Related Classifications

HY-N9447 Related Classifications