CyLip-10
CyLip-10 is a microbial-derived cyclic-lipid antimicrobial peptide. CyLip-10 has broad-spectrum antimicrobial efficacy, low hemolytic activity, and excellent stability. CyLip-10 can disrupt membrane integrity, inhibit biofilm formation and induce membrane permeabilization and bacterial cell death. CyLip-10 reduces bacterial load, promotes wound healing, and alleviates inflammatory responses in a mouse Methicillin (HY-121544)-resistant Staphylococcus aureus skin wound infection model. CyLip-10 can be used for the bacterial infection.
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- Formule: C67H107N17O13
- Masse moléculaire:1358.67
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
CyLip-10 (18-24 h) exhibits broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacterial strains (including S. aureus ATCC 25923, B. subtilis ATCC 23857, E. coli ATCC 25922, K. pneumoniae ATCC 700603, and P. aeruginosa ATCC 27853) with a GMall (the
geometric mean of the MICs) of 7.34 μM[1].
CyLip-10 (1 h) shows low hemolytic toxicity against mouse red blood cells with an HC10 value of >256 μM[1].
CyLip-10 (1 h) has low cytotoxicity against HK-2 cells with a CC50 value of 210 μM[1].
CyLip-10 exhibits good serum stability in mouse serum with a half-life exceeding 48 h[1].
CyLip-10 maintains complete antimicrobial activity against S. aureus and E. coli after 6 h incubation with 2000 μg/mL of trypsin or chymotrypsin[1].
CyLip-10 (8-32 μM; 2-24 h) rapidly kills S. aureus and E. coli and inhibits the formation of biofilms[1].
CyLip-10 has a low propensity to induce resistance in S. aureus and P. aeruginosa over 20 passages[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human kidney proximal tubular epithelial cells (HK-2)
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Concentration:up to 128 μM
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Incubation Time:1 h
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Result:Maintained a cell survival rate exceeding 50% even at the highest concentration of 128 μM.
Achieved a CC50 value of 210 μM.
In Vivo
CyLip-10 (30-100 mg/kg; i.p.; single dose) has lower in vivo toxicity with an LD50 of 49.40 mg/kg in mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/C (male, 6−8 weeks old, 20±2 g, MRSA-induced skin wound infection model)[1]
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Dosage:2 mg/mL
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Administration:Topical; six doses at 1-hour intervals
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Result:Significantly reduced MRSA bacterial load in wounds compared to the model group.
Reduced serum TNF-α levels.
Produced wound areas smaller than the model group by day 4 and nearly healed by day 8.
Improved skin tissue structure with distinct epidermis and dermis layers, less inflammatory cell infiltration, and basically normal cell morphology.
Chemical Information
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Masse moléculaire 1358.67
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Formule C67H107N17O13
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Sequence
C8-{Dab}-{Dab}-{D-Tyr}-Lys-{Dab}-{Dab}-Trp-{D-Leu}-{D-Leu}-Glu (Lactam bridge: Lys4-Glu10)
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Sequence Shortening
C8-{Dab}-{Dab}-{D-Tyr}-K-{Dab}-{Dab}-W-{D-Leu}-{D-Leu}-E (Lactam bridge: Lys4-Glu10)
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CyLip-10 Related Classifications
HY-P11607 Related Classifications
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