ALDH4A1 Protein, Human (sf9)
Based on 1 publication(s) in Google Scholar
The ALDH4A1 protein is a key enzyme that irreversibly converts delta-1-pyrroline-5-carboxylate (P5C) to glutamate, a key step linking the urea and tricarboxylic acid cycles. ALDH4A1 prefers glutamate γ-semialdehyde but also accepts substrates such as succinate, glutaric acid, and adipic acid semialdehyde. ALDH4A1 Protein, Human (sf9) is the recombinant human-derived ALDH4A1 protein, expressed by Sf9 insect cells , with tag free.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ALDH4A1 protein is a key enzyme that irreversibly converts delta-1-pyrroline-5-carboxylate (P5C) to glutamate, a key step linking the urea and tricarboxylic acid cycles. ALDH4A1 prefers glutamate γ-semialdehyde but also accepts substrates such as succinate, glutaric acid, and adipic acid semialdehyde. ALDH4A1 Protein, Human (sf9) is the recombinant human-derived ALDH4A1 protein, expressed by Sf9 insect cells , with tag free.
Background
ALDH4A1, a pivotal enzyme, catalyzes the irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived from either proline or ornithine, into glutamate. This enzymatic transformation serves as a crucial step in the intricate pathway linking the urea and tricarboxylic acid cycles. The preferred substrate for ALDH4A1 is glutamic gamma-semialdehyde, while it also accepts other substrates such as succinic, glutaric, and adipic semialdehydes. The enzymatic activity of ALDH4A1 plays a central role in maintaining the metabolic balance between proline, ornithine, and glutamate, contributing to the efficient utilization of these amino acids within the interconnected cellular pathways.
Publications (1)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Tag Free
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Accession
P30038 (K25-Q563)
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Molecular Construction
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N-term
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ALDH4A1 (K25-Q563)
Accession # P30038 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
ALDH4A1; Mitochondrial Delta-1-Pyrroline 5-Carboxylate Dehydrogenase; Prev. ALDH4; Aldehyde Dehydrogenase 4 Family, Member A1; L-Glutamate Gamma-Semialdehyde Dehydrogenase; Epididymis Secretory Sperm Binding Protein; P5CDh; Multifunctional Fusion Protein;
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AA Sequence
KHTSSLKVANEPVLAFTQGSPERDALQKALKDLKGRMEAIPCVVGDEEVWTSDVQYQVSPFNHGHKVAKFCYADKSLLNKAIEAALAARKEWDLKPIADRAQIFLKAADMLSGPRRAEILAKTMVGQGKTVIQAEIDAAAELIDFFRFNAKYAVELEGQQPISVPPSTNSTVYRGLEGFVAAISPFNFTAIGGNLAGAPALMGNVVLWKPSDTAMLASYAVYRILREAGLPPNIIQFVPADGPLFGDTVTSSEHLCGINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGECGGKNFHFVHRSADVESVVSGTLRSAFEYGGQKCSACSRLYVPHSLWPQIKGRLLEEHSRIKVGDPAEDFGTFFSAVIDAKSFARIKKWLEHARSSPSLTILAGGKCDDSVGYFVEPCIVESKDPQEPIMKEEIFGPVLSVYVYPDDKYKETLQLVDSTTSYGLTGAVFSQDKDVVQEATKVLRNAAGNFYINDKSTGSIVGQQPFGGARASGTNDKPGGPHYILRWTSPQVIKETHKPLGDWSYAYMQ
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Predicted Molecular Mass
59.2 kDa
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Molecular Weight
Approximately 54 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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