IL-16 Protein, Human
Based on 1 Customer Validation
IL-16 Protein, Human is a proinflammatory cytokine containing 130 amino acids.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
IL-16 Protein, Human is a proinflammatory cytokine containing 130 amino acids.
Recombinant Human IL-16 produced in E. coli is a single, non-glycosylated polypeptide chain containing 130 amino acids. Interleukin-16, a proinflammatory cytokine produced in CD8+ lymphocytes, is synthesized as a precursor protein (pro-IL-16). It is postulated that the C-terminal region of pro-IL-16 is cleaved, releasing bioactive IL-16. IL-16 is produced and secreted predominantly by CD8+ T lymphocytes, and it exerts chemoattractant, growth factor, and other activities on lymphocytes, monocytes, and eosinophils by interaction with CD4[1].
Measured by its binding ability in a functional ELISA. Immobilized CD4 at 2 μg/mL (100 μL/well) can bind biotinylated IL-16.The ED50 for this effect is 9.388 ng/mL.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag Tag Free
-
Accession
Q14005-1 (M1203-S1332)
-
Protein Length
Partial
-
Synonyms
IL16; FLJ42735; Interleukin 16; FLJ16806; Pro-Interleukin-16; IL-16; PrIL-16; Interleukin 16 (Lymphocyte Chemoattractant Factor); LCF; Neuronal Interleukin 16; Lymphocyte Chemoattractant Factor; IL16 Protein; Prointerleukin 16; PRIL16; HsT19289; NIL16
-
AA Sequence
MPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS
-
Predicted Molecular Mass
13.4 kDa
-
Molecular Weight
Approximately 16-20 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized after extensive dialysis against 20 mM acetic buffer, pH 5.0, 150 mM NaCl and 0.5 mM DTT or 20 mM PB, 150 mM NaCl, pH 7.0.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (261 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
