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IL-6 Protein, Mouse

31 Cited Publications
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Based on 31 publication(s) in Google Scholar

IL-6 Protein is a multifunctional cytokine secreted by both immune and non-immune cells, and belongs to the interleukin family. IL-6 Protein plays a key role in immune responses, inflammatory reactions and other processes. IL-6 Protein, Mouse is a recombinant IL-6 protein expressed by E. coli without a tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • IL-6 Protein, Mouse Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IL-6 Protein is a multifunctional cytokine secreted by both immune and non-immune cells, and belongs to the interleukin family. IL-6 Protein plays a key role in immune responses, inflammatory reactions and other processes. IL-6 Protein, Mouse is a recombinant IL-6 protein expressed by E. coli without a tag[1][2][3].

Background

IL-6 is a multifunctional cytokine secreted by immune cells (such as T cells, B cells, and macrophages) and non-immune cells (such as fibroblasts, endothelial cells, and hepatocytes). IL-6 transmits signals by binding to its receptor. As a cytokine rapidly and transiently produced in response to infection and tissue damage, IL-6 participates in host defense by stimulating acute-phase responses, hematopoiesis, and immune reactions. Its dysregulation can trigger chronic inflammation and autoimmune diseases. In the initial stage of inflammation, IL-6 is transported via blood to the liver to induce the synthesis of multiple acute-phase proteins. It also participates in regulating serum iron and zinc levels and platelet release. In immune responses, IL-6 promotes Th17 differentiation, inhibits Treg differentiation, and affects immune tolerance[1][2][3].

In Vitro

IL-6 Protein (Mouse; 25 ng/mL; 24 h) can promote the production of inflammatory factors MCP-1, MMP-3, CCL5, CXCL12 and the expression of hypertrophic markers COL10A1 and RUNX2 in primary mouse chondrocytes. The mechanism involves the activation of the JAK2/STAT3 pathway[3].

In Vivo

IL-6 Protein (Mouse; 0.3 μg/kg; intra-articular injection; single dose) can cause spinal hyperexcitability in rats[3].

Verified Bioactivity

1.The ED50 is <0.02 ng/mL as measured by murine 7TD1 cells, corresponding to a specific activity of >5 × 107 units/mg.
2. Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL-6 Protein is immobilized at 5 μg/mL (100 µL/well) can bind Biotinylated Recombinant Mouse IL-6 R alpha (HY-P72538). The ED50 for this effect is 20-156 ng/mL.
3. Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-6 at 5 μg/mL (100 μL/well) on the plate can bind Mouse IL-6R alpha (HY-P72538). The ED50 for this effect is 20-156 ng/mL.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag Tag Free
  • Accession

    P08505 (F25-T211)

  • Gene ID
    16193  [NCBI]
  • Molecular Construction
    • N-term
    • IL-6 (F25-T211)
      Accession # P08505
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    IL6; Hybridoma Growth Factor; Prev. IFNB2; IFN-Beta-2; IL-6; BSF-2; Interleukin-6; CDF; BSF2; Interleukin 6 (Interferon, Beta 2); HGF; B-Cell Differentiation Factor; HSF; Truncated Interleukin 6; B-Cell Stimulatory Factor 2; Interferon, Beta 2; CTL Differ

  • AA Sequence

    FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT

  • Predicted Molecular Mass

    21.8 kDa

  • Molecular Weight

    Approximately 22 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
3.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 500 mM arginine, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Frorath B, et al. Production of recombinant rat interleukin-6 in Escherichia coli using a novel highly efficient expression vector pGEX-3T. Biotechniques. 1992 Apr;12(4):558-63. [Content Brief]

[2]. Tanaka T, et al. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10):a016295. [Content Brief]

[3]. Mima Z, et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. Int Immunopharmacol. 2022 Dec;113(Pt A):109340. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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=
Mass (in vial) Mass (in vial)
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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Mouse IL-6, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-6 R alpha that produces 50% of the optimal binding response is found to be approximately 117.5 ng/mL.

  • 1/16

    IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Jan 30;18(4):3053-3072. [Abstract]

    Customer Validation
     Validation of adsorption capacity of nanomaterials with different concentration gradients on inflammatory factors/LPS/NGF. The remaining concentrations of IL-6 (0-100 μg/mL, 2 h) were detected by an enzyme-linked immunosorbent assay (ELISA), after coculturing with MnO2 nanoparticles, MnO2@MNP nanoparticles, and MnO2@TMNP nanoparticles at different concentrations.

  • 1/16

    IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Apr 13;43(1):112. [Abstract]

    Customer Validation
     sgERO1α NTC/T1 null cells were treated with IL-6 (20 ng/ml), MIP-1α (100 ng/ml), or MIG (100 ng/ml) for 24 h.

  • 1/16

    IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Apr 13;43(1):112. [Abstract]

    Customer Validation
     sgERO1α NTC/T1 null cells were treated with IL-6 (20 ng/ml), MIP-1α (100 ng/ml), or MIG (100 ng/ml) for 24 h.

  • 1/16

    IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Apr 13;43(1):112. [Abstract]

    Customer Validation
     IL-6 (20 ng/ml, 12 h) pre-treated sgERO1α NTC/T1 null cells were transfected with STAT3 siRNAs or control siRNA (siNC) for 48 h. H IL-6 (20 ng/ml, 12 h) pre-treated sgERO1α NTC/T1 null cells were treated with different concentrations of S3I-201 for 24 h.

  • 1/16

    IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Apr 13;43(1):112. [Abstract]

    Customer Validation
     IL-6 (20 ng/ml, 12 h) pre-treated sgERO1α NTC/T1 null cells were transfected with STAT3 siRNAs or control siRNA (siNC) for 48 h. H IL-6 (20 ng/ml, 12 h) pre-treated sgERO1α NTC/T1 null cells were treated with different concentrations of S3I-201 for 24 h.

  • IL-6 Protein, Mouse Product Image
    1/16
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • Experimental Validation Results for IL-6 Protein, Mouse
  • IL-6 Protein, Mouse Product Image

  • IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Jan 30;18(4):3053-3072. [Abstract]

    Validation of adsorption capacity of nanomaterials with different concentration gradients on inflammatory factors/LPS/NGF. The remaining concentrations of IL-6 (0-100 μg/mL, 2 h) were detected by an enzyme-linked immunosorbent assay (ELISA), after coculturing with MnO2 nanoparticles, MnO2@MNP nanoparticles, and MnO2@TMNP nanoparticles at different concentrations.

  • IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Apr 13;43(1):112. [Abstract]

    sgERO1α NTC/T1 null cells were treated with IL-6 (20 ng/ml), MIP-1α (100 ng/ml), or MIG (100 ng/ml) for 24 h.

  • IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Apr 13;43(1):112. [Abstract]

    sgERO1α NTC/T1 null cells were treated with IL-6 (20 ng/ml), MIP-1α (100 ng/ml), or MIG (100 ng/ml) for 24 h.

  • IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Apr 13;43(1):112. [Abstract]

    IL-6 (20 ng/ml, 12 h) pre-treated sgERO1α NTC/T1 null cells were transfected with STAT3 siRNAs or control siRNA (siNC) for 48 h. H IL-6 (20 ng/ml, 12 h) pre-treated sgERO1α NTC/T1 null cells were treated with different concentrations of S3I-201 for 24 h.

  • IL-6 Protein, Mouse purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Apr 13;43(1):112. [Abstract]

    IL-6 (20 ng/ml, 12 h) pre-treated sgERO1α NTC/T1 null cells were transfected with STAT3 siRNAs or control siRNA (siNC) for 48 h. H IL-6 (20 ng/ml, 12 h) pre-treated sgERO1α NTC/T1 null cells were treated with different concentrations of S3I-201 for 24 h.