DBCO-dT Phosphoramidite

DBCO-dT Phosphoramidite enables site-specific, metal-free click chemistry conjugation of two azide-labeled Aβ(14-23) peptides to a homogeneous, flexible FNA tether during automated oligonucleotide synthesis^[1].
DBCO-dT Phosphoramidite-modified FNA tethers enable stable immobilization of Aβ(14-23) peptides that spontaneously form antiparallel dimers, which exhibit a most probable rupture force of 53 pN as measured by AFM force spectroscopy^[1].
DBCO-dT Phosphoramidite (0.1-100 mM; 24 h)-modified oligonucleotides on polystyrene supports achieve high (94-97%) conjugation yields at internal positions near the 3^′-end across all tested labels, while hydrophilic CPG supports drastically reduce yields for lipophilic labels at these positions; a 1 mM modifier concentration is sufficient for efficient conjugation across most conditions^[2].
DBCO-dT Phosphoramidite (0.1-100 mM; 24 h)-modified oligonucleotides achieve ≥90% conjugation yields at the 5′-end across all tested solid supports and concentrations (0.1-100 mM) for AzaPc, ACR, and Cy5, with BDP requiring 1 mM; reaction times for maximal yields range from 30 min to 6 h depending on label polarity, and unreacted BDP can be recycled for three consecutive conjugation cycles without structural degradation and with consistently high yields^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.