Dimethyl pimelimidate dihydrochloride

Name: Dimethyl pimelimidate dihydrochloride
Brand: MedChemExpress
SKU: HY-W123005
Rating: 4.5 (1 reviews)

Cat. No.: HY-W123005 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Dimethyl pimelimidate dihydrochloride (DMP) is a protein crosslinker that can be used in chromatin immunoprecipitation experiments (ChIP) to enhance signal.

For research use only. We do not sell to patients.

Dimethyl pimelimidate dihydrochloride Chemical Structure

CAS No. : 58537-94-3

Size	Price	Stock	Quantity
50 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Purity & Documentation
References
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Description

Dimethyl pimelimidate dihydrochloride (DMP) is a protein crosslinker that can be used in chromatin immunoprecipitation experiments (ChIP) to enhance signal^[1].

Molecular Weight

259.17

Formula

C₉H₂₀Cl₂N₂O₂

CAS No.

58537-94-3

Appearance

Solid

Color

White to off-white

SMILES

N=C(OC)CCCCCC(OC)=N.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (385.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (385.85 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8585 mL	19.2924 mL	38.5847 mL
5 mM	0.7717 mL	3.8585 mL	7.7169 mL
10 mM	0.3858 mL	1.9292 mL	3.8585 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (268 KB) SDS (0 KB)

COA (243 KB) HNMR (131 KB)

Handling Instructions (2659 KB)

References

[1]. Milne TA, et al. Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. Methods Mol Biol. 2009;538:409-23. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.8585 mL	19.2924 mL	38.5847 mL	96.4618 mL
	5 mM	0.7717 mL	3.8585 mL	7.7169 mL	19.2924 mL
	10 mM	0.3858 mL	1.9292 mL	3.8585 mL	9.6462 mL
	15 mM	0.2572 mL	1.2862 mL	2.5723 mL	6.4308 mL
	20 mM	0.1929 mL	0.9646 mL	1.9292 mL	4.8231 mL
	25 mM	0.1543 mL	0.7717 mL	1.5434 mL	3.8585 mL
	30 mM	0.1286 mL	0.6431 mL	1.2862 mL	3.2154 mL
	40 mM	0.0965 mL	0.4823 mL	0.9646 mL	2.4115 mL
	50 mM	0.0772 mL	0.3858 mL	0.7717 mL	1.9292 mL
	60 mM	0.0643 mL	0.3215 mL	0.6431 mL	1.6077 mL
	80 mM	0.0482 mL	0.2412 mL	0.4823 mL	1.2058 mL
	100 mM	0.0386 mL	0.1929 mL	0.3858 mL	0.9646 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.