Dimethyl pimelimidate dihydrochloride

Name: Dimethyl pimelimidate dihydrochloride
Brand: MedChemExpress
SKU: HY-W123005
Rating: 4.5 (1 reviews)

Cat. No.: HY-W123005 Purity: 98.0%: Data Sheet
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Dimethyl pimelimidate dihydrochloride is a bifunctional imidoester protein-protein crosslinker used in chromatin immunoprecipitation (ChIP) assays.

For research use only. We do not sell to patients.

Dimethyl pimelimidate dihydrochloride Chemical Structure

CAS No. : 58537-94-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Dimethyl pimelimidate dihydrochloride

•Drug Metab Dispos. 2025 Apr;53(4):100057. [Abstract]

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Biological Activity
Purity & Documentation
References
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Description

Dimethyl pimelimidate dihydrochloride is a bifunctional imidoester protein-protein crosslinker used in chromatin immunoprecipitation (ChIP) assays^[1]^[2].

In Vitro

Guidelines (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs)^[1].
An alternative fixation protocol using Dimethyl pimelimidate dihydrochloride.
Experimental Procedures:
1. Remove a vial of Dimethyl pimelimidate dihydrochloride from the refrigerator, allow it to warm to room temperature before opening (this substance is highly hygroscopic).
2. Gently rinse cells with PBS, then fix the cells directly in the culture dish or transfer them to a 1.5 mL centrifuge tube, followed by shaking for fixation at room temperature for 1 hour (use 5 mL Dimethyl pimelimidate dihydrochloride/DMSO/PBS for a 10 cm culture dish, and 400 μL Dimethyl pimelimidate dihydrochloride/DMSO/PBS for a 1.5 mL centrifuge tube).
3. Add 1% paraformaldehyde (dissolved in PBS) directly to the well plate/tube to achieve a final paraformaldehyde concentration of 2/3% (for example, add 10 mL of 1% paraformaldehyde to 5 mL of DMP solution, or add 800 μL of paraformaldehyde to 400 μL of solution).
4. Seal the plate with Parafilm, or incubate the samples in 1.5 mL tubes with rotation at room temperature in a fume hood for 5-20 hours (the incubation time is determined based on experimental results for different proteins).
5. Centrifuge at 364 × g for 5 minutes, then rinse the cells with PBS, taking care not to leave sample residues on the tube wall.
6. Add lysis buffer and initiate the ChIP assay. Alternatively, the pellet can be stored frozen at this point.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

259.17

Formula

C₉H₂₀Cl₂N₂O₂

CAS No.

58537-94-3

Appearance

Solid

Color

White to off-white

SMILES

N=C(OC)CCCCCC(OC)=N.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

TFA : 100 mg/mL (385.85 mM; Need ultrasonic)

H₂O : 100 mg/mL (385.85 mM; Need ultrasonic)

DMSO : 100 mg/mL (385.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8585 mL	19.2924 mL	38.5847 mL
5 mM	0.7717 mL	3.8585 mL	7.7169 mL
10 mM	0.3858 mL	1.9292 mL	3.8585 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

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Data Sheet (271 KB) SDS (393 KB)

COA (243 KB) HNMR (131 KB)

Handling Instructions (2659 KB)

References

[1]. Milne TA, et al. Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. Methods Mol Biol. 2009;538:409-23. [Content Brief]

[2]. Smith TJ, et al. Investigation of the effects of crosslinking glutamate dehydrogenase with dimethyl pimelimidate. Arch Biochem Biophys. 1985;239(1):63-73. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

TFA / H₂O / DMSO	1 mM	3.8585 mL	19.2924 mL	38.5847 mL	96.4618 mL
	5 mM	0.7717 mL	3.8585 mL	7.7169 mL	19.2924 mL
	10 mM	0.3858 mL	1.9292 mL	3.8585 mL	9.6462 mL
	15 mM	0.2572 mL	1.2862 mL	2.5723 mL	6.4308 mL
	20 mM	0.1929 mL	0.9646 mL	1.9292 mL	4.8231 mL
	25 mM	0.1543 mL	0.7717 mL	1.5434 mL	3.8585 mL
	30 mM	0.1286 mL	0.6431 mL	1.2862 mL	3.2154 mL
	40 mM	0.0965 mL	0.4823 mL	0.9646 mL	2.4115 mL
	50 mM	0.0772 mL	0.3858 mL	0.7717 mL	1.9292 mL
	60 mM	0.0643 mL	0.3215 mL	0.6431 mL	1.6077 mL
	80 mM	0.0482 mL	0.2412 mL	0.4823 mL	1.2058 mL
	100 mM	0.0386 mL	0.1929 mL	0.3858 mL	0.9646 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.