Endoglycoceramidase II (EGCase II)

Purified recombinant Endoglycoceramidase II (EGCase II) from Rhodococcus sp. (153 nM; 1 h) exhibits optimal enzymatic activity at pH 5.5, hydrolyzing 4MU-Lac in a time-dependent manner^[1].
Purified recombinant Endoglycoceramidase II (EGCase II) from Rhodococcus sp. (5 μM) is thermolabile, with an apparent melting temperature of 42 °C at a 1 °C/min heating rate, a half-life of ~10 min at 37 °C, and kinetic stability at 25 °C^[1].
Purified recombinant Endoglycoceramidase II (EGCase II) from Rhodococcus sp. is susceptible to tryptic digestion at 25 °C and 37 °C, with cleavage at Arg321/Arg332, but becomes resistant to digestion in the presence of lauryl glucoside or Triton X-100^[1].
Purified recombinant Endoglycoceramidase II (EGCase II) from Rhodococcus sp. (1 mg/mL) exists in an equilibrium between dimeric (100 kDa) and monomeric (50 kDa) states in solution, with both forms exhibiting equivalent enzymatic activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.