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Cat. No.: HY-DY2022

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Eosin-Nigrosin Sperm Viability Staining Solution

Eosin-Nigrosin Sperm Viability Staining Solution is a differential viability staining solution used to distinguish live/dead sperm and other cells, consisting of Eosin Y (HY-D0505) and Nigrosin (HY-W250145). Eosin-Nigrosin Sperm Viability Staining Solution cannot penetrate the membrane of live cells; it stains dead cells dark pink and uses nigrosin to form a contrasting dark background. Eosin-Nigrosin Sperm Viability Staining Solution can distinguish live sperm, dead sperm and sperm with neck leakage, and can stain dead sperm or sperm affected by excessive microbial growth.

For research use only. We do not sell to patients.

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Please store the product under the recommended conditions in the Certificate of Analysis.

Eosin-Nigrosin Sperm Viability Staining Solution Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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Description

In Vitro

Eosin-Nigrosin Sperm Viability Staining Solution relies on the property that Eosin cannot penetrate the intact cell membrane of live sperm to clearly distinguish the viability status of bovine and human sperm, bacteria and other somatic cells; live sperm exhibit white/pale pink heads, dead sperm show dark pink/red-stained heads, and sperm with only partial staining at the neck are determined to be live sperm^[1]^[2].
Eosin-Nigrosin Sperm Viability Staining Solution enables rapid preparation of slides for sperm viability counting, and the stained slides can be preserved for a long period^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Agarwal A, et al. Eosin‑Nigrosin Staining Procedure. In: Agarwal A, Gupta S, Sharma R (eds). Andrological Evaluation of Male Infertility. Springer, Cham, 2016.

[2]. Williams W W. Study of Sperm Vitality with the Aid of Eosin‑Nigrosin Stain. Fertility and Sterility, 1950, 1(2):178‑181.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Eosin-Nigrosin Sperm Viability Staining Solution

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Eosin-Nigrosin Sperm Viability Staining Solution

Biological Activity

Description

In Vitro

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Eosin-Nigrosin Sperm Viability Staining Solution Related Classifications

HY-DY2022 Related Classifications

Bioz

Keywords

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