Ergosterol peroxide

Cat. No.: HY-N3845 Purity: 99.57%: Data Sheet
COA

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Ergosterol peroxide is a steroid derivative and can be isolated from a variety of fungi, yeast, lichens or sponges. Ergosterol peroxide has anti-tumour, proapoptotic, anti-inflammatory, anti-mycobacterial, and anti-proliferative activities.

For research use only. We do not sell to patients.

Ergosterol peroxide Chemical Structure

CAS No. : 2061-64-5

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Ergosterol peroxide

•Lett Drug Des Discov. 2026 Feb 11.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	14.32 μg/mL Compound: 5	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 23511021]
B16	IC₅₀	77.9 μM Compound: 6	Cytotoxicity against murine B16 cells by MTT assay Cytotoxicity against murine B16 cells by MTT assay	[PMID: 17256903]
BV-2	IC₅₀	6.3 μM Compound: 22	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method	[PMID: 21028898]
HEK293	EC₅₀	0.85 μM Compound: 2	Agonist activity at full length mouse FXR/RXRalpha expressed in human HEK293 cells assessed as induction of transcriptional activity after 18 hrs by dual luciferase reporter gene assay Agonist activity at full length mouse FXR/RXRalpha expressed in human HEK293 cells assessed as induction of transcriptional activity after 18 hrs by dual luciferase reporter gene assay	[PMID: 22014750]
HeLa	IC₅₀	19.54 μM Compound: Ergosterol peroxide	Cytotoxicity against human HeLa cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay	[PMID: 38107170]
Hep 3B2	IC₅₀	16.51 μg/mL Compound: 5	Cytotoxicity against human Hep3B cells Cytotoxicity against human Hep3B cells	[PMID: 23511021]
HepG2	IC₅₀	15.27 μg/mL Compound: 5	Cytotoxicity against human HepG2 cells Cytotoxicity against human HepG2 cells	[PMID: 23511021]
HepG2	IC₅₀	23.35 μM Compound: Ergosterol peroxide	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 38107170]
KB	ED₅₀	9.79 μg/mL Compound: 4	Cytotoxicity against human KB cells after 4 days Cytotoxicity against human KB cells after 4 days	[PMID: 1791484]
MCF7	IC₅₀	13.41 μg/mL Compound: 5	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 23511021]
MCF7	IC₅₀	18 μM Compound: Ergosterol peroxide	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth by CCK8 assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth by CCK8 assay	[PMID: 38107170]
MDA-MB-231	IC₅₀	16.74 μg/mL Compound: 5	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 23511021]
PLC-PRF-5	ED₅₀	10.99 μg/mL Compound: 4	Cytotoxicity against human PLC/PRF/5 cells after 4 days Cytotoxicity against human PLC/PRF/5 cells after 4 days	[PMID: 1791484]
RAW264.7	IC₅₀	28.7 μM Compound: 117	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production	[PMID: 31255927]
RAW264.7	IC₅₀	9.4 μM Compound: 9	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by Griess reaction method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by Griess reaction method	[PMID: 23357630]
SNU-354	IC₅₀	158.2 μM Compound: 231	Cytotoxicity against human SNU354 cells Cytotoxicity against human SNU354 cells	[PMID: 17618015]
SNU-C4	IC₅₀	84.6 μM Compound: 231	Cytotoxicity against human SNUC4 cells Cytotoxicity against human SNUC4 cells	[PMID: 17618015]
SNU1	IC₅₀	18.7 μM Compound: 231	Cytotoxicity against human SNU1 cells Cytotoxicity against human SNU1 cells	[PMID: 17618015]

Molecular Weight

428.65

Formula

C₂₈H₄₄O₃

CAS No.

2061-64-5

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]3([H])[C@]4(C=C[C@]1(C[C@H](CC2)O)OO4)[C@@]5([H])[C@](CC3)([C@@](CC5)([H])[C@H](C)/C=C/[C@H](C)C(C)C)C

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 11.11 mg/mL (25.92 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3329 mL	11.6645 mL	23.3291 mL
5 mM	0.4666 mL	2.3329 mL	4.6658 mL
10 mM	0.2333 mL	1.1665 mL	2.3329 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (269 KB) SDS (393 KB)

COA (249 KB) RP-HPLC (239 KB)

Handling Instructions (2659 KB)

References

[1]. Ming Bu, et al. Synthesis of Ergosterol Peroxide Conjugates as Mitochondria Targeting Probes for Enhanced Anticancer Activity. Molecules. 2019 Sep 11;24(18):3307 [Content Brief]

[2]. Taotao Ling, et al. Development of ergosterol peroxide probes for cellular localisation studies. Org Biomol Chem. 2019 May 29;17(21):5223-5229. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3329 mL	11.6645 mL	23.3291 mL	58.3226 mL
	5 mM	0.4666 mL	2.3329 mL	4.6658 mL	11.6645 mL
	10 mM	0.2333 mL	1.1665 mL	2.3329 mL	5.8323 mL
	15 mM	0.1555 mL	0.7776 mL	1.5553 mL	3.8882 mL
	20 mM	0.1166 mL	0.5832 mL	1.1665 mL	2.9161 mL
	25 mM	0.0933 mL	0.4666 mL	0.9332 mL	2.3329 mL