Fmoc-PNA-J(Bhoc)-OH

Application example of Fmoc-PNA-J (Bhoc)-OH^[3]:
PNAs (2 μM) incorporating γ-hydroxymethyl-modified monomers can form duplexes with complementary DNA, and the position of the modified monomer determines the secondary structure of the resulting duplex; the duplexes formed by PNAs modified at the C-terminus, in the middle, or at both positions differ in structure from those of N-terminally modified PNA/DNA duplexes^[3].
PNAs incorporated with γ-hydroxymethyl-modified monomers (50-100 ng/reaction; 40 min) can bind to double-stranded Sp1 target DNA via strand invasion; the C-terminally modified PNA-C exhibits the highest binding efficiency, while modification at the middle region or simultaneous modification at the middle and C-terminal regions reduces binding activity, and the N-terminally modified PNA-N shows comparable binding efficiency to unmodified PNA^[3].
PNAs (50-200 ng/reaction) incorporated with γ-hydroxymethyl-modified monomers inhibit de novo formation of the Sp1/DNA complex after pre-incubation with target double-stranded DNA; among them, the C-terminally modified PNA-C shows the highest inhibitory efficiency, which is comparable to that of unmodified PNA^[3].
PNAs incorporated with γ-hydroxymethyl-modified monomers (50-400 ng/reaction) do not dissociate pre-formed Sp1/DNA complexes, even at high concentrations^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.