Fmoc-PNA-J(Bhoc)-OH 

Fmoc-PNA-J (Bhoc)-OH is a protected peptide nucleic acid (PNA) monomer building block used for solid-phase synthesis of PNA oligomers. Fmoc-PNA-J (Bhoc)-OH carries Fmoc/Bhoc protecting groups and functions as a standard Fmoc-protected PNA monomer^[1][2][3].

Application example of Fmoc-PNA-J (Bhoc)-OH^[3]:
PNAs (2 μM) incorporating γ-hydroxymethyl-modified monomers can form duplexes with complementary DNA, and the position of the modified monomer determines the secondary structure of the resulting duplex; the duplexes formed by PNAs modified at the C-terminus, in the middle, or at both positions differ in structure from those of N-terminally modified PNA/DNA duplexes^[3].
PNAs incorporated with γ-hydroxymethyl-modified monomers (50-100 ng/reaction; 40 min) can bind to double-stranded Sp1 target DNA via strand invasion; the C-terminally modified PNA-C exhibits the highest binding efficiency, while modification at the middle region or simultaneous modification at the middle and C-terminal regions reduces binding activity, and the N-terminally modified PNA-N shows comparable binding efficiency to unmodified PNA^[3].
PNAs (50-200 ng/reaction) incorporated with γ-hydroxymethyl-modified monomers inhibit de novo formation of the Sp1/DNA complex after pre-incubation with target double-stranded DNA; among them, the C-terminally modified PNA-C shows the highest inhibitory efficiency, which is comparable to that of unmodified PNA^[3].
PNAs incorporated with γ-hydroxymethyl-modified monomers (50-400 ng/reaction) do not dissociate pre-formed Sp1/DNA complexes, even at high concentrations^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

701.72

C₃₉H₃₅N₅O₈

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Petkowski JJ, et al. Astrobiological implications of the stability and reactivity of peptide nucleic acid (PNA) in concentrated sulfuric acid. Sci Adv. 2025;11(13):eadr0006.  [Content Brief]

  [2]. Polak A, et al. The Application of Peptide Nucleic Acids (PNA) in the Inhibition of Proprotein Convertase Subtilisin/Kexin 9 (PCSK9) Gene Expression in a Cell-Free Transcription/Translation System. Int J Mol Sci. 2024;25(3):1463. Published 2024 Jan 25.  [Content Brief]

  [3]. Pensato S, et al. gamma-Hydroxymethyl PNAs: Synthesis, interaction with DNA and inhibition of protein/DNA interactions. Bioorg Chem. 2010;38(5):196-201.  [Content Brief]