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  4. γ-Hexalactone

γ-Hexalactone  (Synonyms: γ-Caprolactone)

Cat. No.: HY-W015892 Purity: 99.46%
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γ-Hexalactone (γ-Caprolactone) is a gamma-lactone found in ripe fruits. γ-Hexalactone induces DNA damage in human lymphocytes and HepG2 cells, modulates cytokine secretion in human lymphocytes, and reduces recombinant PON1 activity. γ-Hexalactone serves as a dose-dependent oviposition inhibitor against Bactrocera oleae. γ-Hexalactone can be used for the research of Bactrocera oleae pest management.

For research use only. We do not sell to patients.

γ-Hexalactone

γ-Hexalactone Chemical Structure

CAS No. : 695-06-7

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Liquid
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of γ-Hexalactone:

γ-Hexalactone Related Antibodies

Top Publications Citing Use of Products

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

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TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

γ-Hexalactone (γ-Caprolactone) is a gamma-lactone found in ripe fruits. γ-Hexalactone induces DNA damage in human lymphocytes and HepG2 cells, modulates cytokine secretion in human lymphocytes, and reduces recombinant PON1 activity. γ-Hexalactone serves as a dose-dependent oviposition inhibitor against Bactrocera oleae. γ-Hexalactone can be used for the research of Bactrocera oleae pest management[1][2][3][4][5].

IC50 & Target[2]

IL-1

 

IL-6

 

IL-10

 

TNF-α

 

In Vitro

γ-Hexalactone (10-100 mM; 3 h) induces minimal DNA damage alone in HepG2 cells, but co-incubation with rPON1 drastically increases DNA damage at 100 mM[1].
γ-Hexalactone (0.0515-5.15 μM; 72 h) induces significant DNA damage in cultured human peripheral blood lymphocytes at 5.15 μM[2].
γ-Hexalactone (0.0515-5.15 μM; 72 h) exhibits immunomodulatory effects in cultured human peripheral blood lymphocytes, increasing proinflammatory cytokine IL-1 levels at all tested concentrations, and further increasing IL-6, TNF-α, IFN-γ, and decreasing IL-10 levels at the highest tested concentration of 5.15 μM[2].
γ-Hexalactone (0.0515-5.15 μM; 72 h) displays no obvious cytotoxicity in cultured human lymphocytes[2].
γ-Hexalactone (1.0 mg; 24 h) significantly reduces oviposition activity of female Bactrocera oleae after mating, with a mean oviposition activity index (OAI) of -0.56[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

γ-Hexalactone Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: human peripheral blood lymphocytes
Concentration: 0.0515 μM, 0.515 μM, 5.15 μM
Incubation Time: 72 h
Result: Maintains total lymphocyte counts similar to the negative control across all tested concentrations.
Keeps lymphocyte viability above 96% across all tested concentrations.
Exhibits no significant cytotoxicity.

ELISA Assay[2]

Cell Line: cultured human peripheral blood lymphocytes
Concentration: 0.0515 μM, 0.515 μM, 5.15 μM
Incubation Time: 72 h
Result: Upregulated IL‑1 levels at all tested concentrations, elevated IL‑6, TNF‑α, and IFN‑γ levels while reducing IL‑10 levels at 5.15 μM, and exerted no significant effects on IL‑6, TNF‑α, IFN‑γ, or IL‑10 levels at 0.515 μM or 0.0515 μM.
In Vivo

γ-Hexalactone (30-1000 mg/kg/day; p.o.; daily; 28 days) has a repeated dose toxicity No Observed Adverse Effect Level (NOAEL) of 1000 mg/kg/day in Sprague Dawley rats, with adaptive liver changes noted at the highest dose[4].
γ-Hexalactone (100-1000 mg/kg/day; p.o.; daily; gestation days 6-19) has a developmental toxicity NOAEL of 300 mg/kg/day in pregnant Sprague Dawley rats, with no adverse maternal effects and only isolated, non-dose-related fetal changes[4].
γ-Hexalactone (3.5% intradermal; 100% topical) does not induce skin sensitization in Hartley guinea pigs when tested via intradermal and topical exposure in a maximization test[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague Dawley (both sexes, 5 rats/sex/dose)[4]
Dosage: 30 mg/kg/day; 100 mg/kg/day; 300 mg/kg/day; 1000 mg/kg/day
Administration: p.o.; daily; 28 days
Result: Showed no treatment‑related mortality or body weight loss, induced low‑incidence only at the highest dose, increased liver weights reversibly in a high‑dose‑dependent manner, caused mild reversible hepatic changes regarded as adaptive.
Animal Model: Sprague Dawley (female, 25 rats/dose, pregnant)[4]
Dosage: 100 mg/kg/day; 300 mg/kg/day; 1000 mg/kg/day
Administration: p.o.; daily; gestation days 6-19
Result: Showed no treatment-related changes in maternal animals.
Significantly decreased fetal body weight at 1000 mg/kg/day within historical control ranges.
Caused isolated fetal malformations at 300 mg/kg/day with no dose-related tendency.
Produced fetal skeletal variants within historical control ranges unrelated to treatment.
Molecular Weight

114.14

Formula

C6H10O2
CAS No.
Appearance

Liquid (Density: 1.023  g/cm3  )

Color

Colorless to light yellow

SMILES

O=C1OC(CC)CC1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (876.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.7612 mL 43.8059 mL 87.6117 mL
5 mM 1.7522 mL 8.7612 mL 17.5223 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (21.90 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (21.90 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.77%

SDS (396 KB)

COA (248 KB) HNMR (118 KB) GC (206 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 8.7612 mL 43.8059 mL 87.6117 mL 219.0293 mL
5 mM 1.7522 mL 8.7612 mL 17.5223 mL 43.8059 mL
10 mM 0.8761 mL 4.3806 mL 8.7612 mL 21.9029 mL
15 mM 0.5841 mL 2.9204 mL 5.8408 mL 14.6020 mL
20 mM 0.4381 mL 2.1903 mL 4.3806 mL 10.9515 mL
25 mM 0.3504 mL 1.7522 mL 3.5045 mL 8.7612 mL
30 mM 0.2920 mL 1.4602 mL 2.9204 mL 7.3010 mL
40 mM 0.2190 mL 1.0951 mL 2.1903 mL 5.4757 mL
50 mM 0.1752 mL 0.8761 mL 1.7522 mL 4.3806 mL
60 mM 0.1460 mL 0.7301 mL 1.4602 mL 3.6505 mL
80 mM 0.1095 mL 0.5476 mL 1.0951 mL 2.7379 mL
100 mM 0.0876 mL 0.4381 mL 0.8761 mL 2.1903 mL
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γ-Hexalactone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

γ-Hexalactone695-06-7γ-CaprolactoneInterleukin RelatedTNF ReceptorInsecticideILTumor Necrosis Factor ReceptorTNFRPON1IL-6Bactrocera oleaeHepG2 cellsIFN-γIL-10TNF-αSprague Dawley ratsIL-1human lymphocytesInhibitorinhibitorinhibit

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