H-Arg-NH2 hydrochloride
Based on 1 Customer Validation
H-Arg-NH2 hydrochloride is a nucleophilic reagent. H-Arg-NH2 hydrochloride can be used in experiments related to the detection of enzyme activity centers.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 14975-30-5
- Formula: C6H17Cl2N5O
- Molecular Weight:246.14
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
Biological Activity
H-Arg-NH₂ (400 mM) hydrochloride and 10 mM Bz-L-Tyr-pNA demonstrats protease synthetic activity in a 90% acetonitrile-water mixture [3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 14975-30-5
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Appearance Solid
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Molecular Weight 246.14
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Formula C6H17Cl2N5O
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Color White to off-white
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SMILES
O=C([C@H](CCCNC(N)=N)N)N.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
H2O : ≥ 100 mg/mL (406.27 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
[1]. K Breddam, et al. Amidation of growth hormone releasing factor (1-29) by serine carboxypeptidase catalysed transpeptidation. Int J Pept Protein Res. 1991, 37, 2. [Content Brief]
[2]. Y Collette, et al. An improved synthesis of an umbelliferyl 5-thioxylopyranoside, precursor of the antithrombotic drug Iliparcil. Carbohydr Res. 1999, 318, 1-4. [Content Brief]
[3]. Sareen R, et al. A microtiter plate assay for the determination of the synthetic activity of protease. Anal Biochem. 2004 Oct 1;333(1):193-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.0627 mL | 20.3136 mL | 40.6273 mL | 101.5682 mL |
| 5 mM | 0.8125 mL | 4.0627 mL | 8.1255 mL | 20.3136 mL | |
| 10 mM | 0.4063 mL | 2.0314 mL | 4.0627 mL | 10.1568 mL | |
| 15 mM | 0.2708 mL | 1.3542 mL | 2.7085 mL | 6.7712 mL | |
| 20 mM | 0.2031 mL | 1.0157 mL | 2.0314 mL | 5.0784 mL | |
| 25 mM | 0.1625 mL | 0.8125 mL | 1.6251 mL | 4.0627 mL | |
| 30 mM | 0.1354 mL | 0.6771 mL | 1.3542 mL | 3.3856 mL | |
| 40 mM | 0.1016 mL | 0.5078 mL | 1.0157 mL | 2.5392 mL | |
| 50 mM | 0.0813 mL | 0.4063 mL | 0.8125 mL | 2.0314 mL | |
| 60 mM | 0.0677 mL | 0.3386 mL | 0.6771 mL | 1.6928 mL | |
| 80 mM | 0.0508 mL | 0.2539 mL | 0.5078 mL | 1.2696 mL | |
| 100 mM | 0.0406 mL | 0.2031 mL | 0.4063 mL | 1.0157 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.