2. Cell Cycle/DNA Damage Epigenetics
  3. HDAC
  4. HDAC6-IN-13

HDAC6-IN-13 (Compound 35m) is a potent, highly selective, orally active HDAC6 inhibitor with an IC50 of 0.019 μM. HDAC6-IN-13 also inhibits HDAC1, HDAC2 and HDAC3 with IC50s of 1.53, 2.06 and 1.03 μM, respectively. HDAC6-IN-13 shows significant BBB permeability and anti-inflammatory activity.

For research use only. We do not sell to patients.

HDAC6-IN-13 Chemical Structure

HDAC6-IN-13 Chemical Structure

CAS No. : 2837128-41-1

Size Price Stock Quantity
5 mg USD 450 In-stock
10 mg USD 720 In-stock
25 mg USD 1440 In-stock
50 mg USD 2300 In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

HDAC6-IN-13 Related Antibodies

Top Publications Citing Use of Products

View All HDAC Isoform Specific Products:

View All Isoforms
HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HDAC6-IN-13 (Compound 35m) is a potent, highly selective, orally active HDAC6 inhibitor with an IC50 of 0.019 μM. HDAC6-IN-13 also inhibits HDAC1, HDAC2 and HDAC3 with IC50s of 1.53, 2.06 and 1.03 μM, respectively. HDAC6-IN-13 shows significant BBB permeability and anti-inflammatory activity[1].

IC50 & Target[1]

HDAC6

0.019 μM (IC50)

HDAC3

1.03 μM (IC50)

HDAC1

1.53 μM (IC50)

HDAC2

2.06 μM (IC50)

In Vitro

HDAC6-IN-13 (Compound 35m) (0.1-1 μM; 24 h) is highly selective toward HDAC6 versus class I HDACs[1].
HDAC6-IN-13 is a slow-on and slow-off tight-binding HDAC6 inhibitor, while exhibits fast-on properties for HDAC1, 2, and 3[1].
HDAC6-IN-13 (5-20 μM; 8 h) shows anti-inflammatory activity in vitro[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HDAC6-IN-13 Related Antibodies

Western Blot Analysis[1]

Cell Line: MV4−11 and J774A.1
Concentration: 0.1, 0.2, 0.5 and 1 μM
Incubation Time: 24 h
Result: Concentration-related accumulation of acetylated tubulin (Ac-Tubulin) was observed, while upregulation of acetylated histone H3 (AcHH3) and acetylated histone H4 (AcHH4) was not apparent even at the concentration of 1 μM.

Western Blot Analysis[1]

Cell Line: J774A.1 cells
Concentration: 5, 10 and 20 μM
Incubation Time: 8 h
Result: Inhibited the cleavage of pro-caspase 1 to p20 in a dose-dependent manner, inhibited the interaction between HDAC6 and dynein.
In Vivo

HDAC6-IN-13 (Compound 35m) (20 mg/kg; p.o. and i.p.; once) displays a remarkable inhibition in LPS-induced inflammation in mice[1].
HDAC6-IN-13 (20 mg/kg; p.o.; once) shows very high oral bioavailability (F% = 93.4%) and significant BBB permeability in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 WT mice, LPS-induced endotoxic shock model[1]
Dosage: 20 mg/kg
Administration: PO and IP, immediately after the LPS injection
Result: Significantly decreased the serum IL-1β levels in LPS-induced mice via both ip and po administration.
Animal Model: Male CD-1 mice[1]
Dosage: 5 mg/kg or 20 mg/kg
Administration: IV (5 mg/kg) or PO (20 mg/kg) (Pharmacokinetic Study)
Result: Pharmacokinetics Characterization of HDAC6-IN-13 (Compound 35m) with iv and Oral Administrationa[1]
PK parameters HDAC6-IN-13 HDAC6-IN-13
administered dose (mg/kg) iv at 5 mg/kg oral at 20 mg/kg
Cmax (ng/mL) 4604 ± 551 5570 ± 551
t1/2 (h) 7.95 ± 0.370 6.80 ± 0.145
AUC0−inf (ng•h/mL) 2755 ± 395 10292 ± 1385
F% n/a 93.4 ± 12.6

aHDAC6-IN-13 was administrated via iv and po (n = 3). The blood sample was collected at different time points after dosing, and the plasma concentration of HDAC6-IN-13 was determined via LC-MS/MS. The area under the plasma concentration versus time curve (AUC) was calculated using the linear trapezoidal method. The pharmacokinetic parameters were obtained using the noncompartmental method. Data are shown as mean ± SD.
Molecular Weight

370.45

Formula

C23H22N4O
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CCNNC(C1=CC=C(C=C1)CN2C3=CC=CC=C3C=CC4=C2C=NC=C4)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (337.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6994 mL 13.4971 mL 26.9942 mL
5 mM 0.5399 mL 2.6994 mL 5.3988 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6994 mL 13.4971 mL 26.9942 mL 67.4855 mL
5 mM 0.5399 mL 2.6994 mL 5.3988 mL 13.4971 mL
10 mM 0.2699 mL 1.3497 mL 2.6994 mL 6.7485 mL
15 mM 0.1800 mL 0.8998 mL 1.7996 mL 4.4990 mL
20 mM 0.1350 mL 0.6749 mL 1.3497 mL 3.3743 mL
25 mM 0.1080 mL 0.5399 mL 1.0798 mL 2.6994 mL
30 mM 0.0900 mL 0.4499 mL 0.8998 mL 2.2495 mL
40 mM 0.0675 mL 0.3374 mL 0.6749 mL 1.6871 mL
50 mM 0.0540 mL 0.2699 mL 0.5399 mL 1.3497 mL
60 mM 0.0450 mL 0.2250 mL 0.4499 mL 1.1248 mL
80 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8436 mL
100 mM 0.0270 mL 0.1350 mL 0.2699 mL 0.6749 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

HDAC6-IN-13 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HDAC6-IN-132837128-41-1HDACHistone deacetylasesAlzheimer’s diseaseanti-inflammatory activityorally activeBBB permeabilityInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
HDAC6-IN-13
Cat. No.:
HY-151261
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.