HDAC6-IN-15
HDAC6-IN-15 is a selective histone deacetylase 6 (HDAC6) inhibitor. HDAC6-IN-15 has potent inhibitory activity for HDAC6 with IC50 value of 38.2 nM. HDAC6-IN-15 can be used for the research of cancer and neurodegenerative diseases.
For research use only. We do not sell to patients.
- Formula: C25H28FFeN3O2
- Molecular Weight:477.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
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HDAC6 38.2 nM (IC50) |
In Vitro
HDAC6-IN-15 (Compound II-5) has potent inhibitory activity for HDAC6 with IC50 value of 38.2 nM[1].
HDAC6-IN-15 (50 μL; 48 h) has antitumor activity against 22RV1, MM1.S, MV4-11, JEKO-1 and 4T1 cells with IC50 value of 8.90 μM, 11.90 μM, 7.83 μM, 4.80 μM and 16.51 μM, respectively[1].
HDAC6-IN-15 (100, 200, 400, 800 nM; 24 h) dose-dependently induces accumulation of acetylated a-tubulin[1].
HDAC6-IN-15 (5, 10 μM; 24 h) can induce cellular apoptosis[1].
HDAC6-IN-15 (4 mg/mL; 48 h) demonstrates an optimal profile on human plasma stability[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:22RV1, MM1.S, MV4-11, JEKO-1 and 4T1 cells
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Concentration:50 μL
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Incubation Time:48 h
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Result:Showed moderate anti-proliferative activities in all the cancer cell lines.
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Cell Line:JEKO-1 cells; 4T1 cells
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Concentration:100, 200, 400, 800 nM; 5, 10 μM
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Incubation Time:24 h
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Result:Significantly increase the levels of acetylated α-tubulin in a concentration dependent manner.
Slightly increased the levels of histone H3 and H4 acetylation.
Significantly increased the ratio of acetylated α-tubulin at the concentration of 800 nM.
Dramatically increased the levels of cleavage of PARP and caspase-3 in cells dose-dependently.
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Cell Line:4T1 cells
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Concentration:5, 10 μM
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Incubation Time:24 h
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Result:Triggered apoptosis in 4T1 cells in a dose-dependent manner, in particularly undergoing early stage apoptosis upon 18 h treatment.
Chemical Information
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Molecular Weight 477.35
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Formula C25H28FFeN3O2
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SMILES
[][Fe][].FC(C=C(C(NO)=O)C=C1)=C1CN(CC2)CCN2C(CCc3cccc3)c4cccc4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HDAC6-IN-15 Related Classifications
HY-152235 Related Classifications
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