HDAC6-IN-75
Based on 1 Customer Validation
HDAC6-IN-75 is a selective HDAC6 inhibitor with an IC50 of 0.17 nM against HDAC6. HDAC6-IN-75 induces the accumulation of acetylated α-tubulin in glioma cells. HDAC6-IN-75 triggers cell cycle changes, increases the SubG1 cell population, and promotes apoptosis in glioma cells and glioblastoma stem cells. HDAC6-IN-75 is applicable for glioma-related research.
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- Pureté : 99.86%
- CAS No.: 400078-78-6
- Formule: C13H12N2O4S
- Masse moléculaire:292.31
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
HDAC6 0.17 nM () |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Cancer cell lines | EC50 |
5 μM
Compound: 7a
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Concentration of compound required for acetylation of histone-4 in human T24 cancer cells
Concentration of compound required for acetylation of histone-4 in human T24 cancer cells
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[PMID: 11597413] |
In Vitro
HDAC6-IN-75 (compound 3a) (0.0032-50 μM; 24-72 h) exhibits time-dependent cytotoxicity in glioma cell lines, with GI50 values ranging from 0.8 μM to 5.3 μM after 72 h of incubation, and induces total growth inhibition in HOG, T98G, and U251MG cells at 72 h[1].
HDAC6-IN-75 (compound 3a) (34.0 μM HOG, 16.2 μM T98G, 50 μM U87MG, 28.9 μM U251MG; 24 h) increases acetyl-α-tubulin levels in all four tested glioma cell lines and acetyl-histone H3 levels in HOG, T98G, and U251MG cells after 24 h of treatment, consistent with its HDAC6 inhibitory activity and concurrent class I HDAC inhibition at cellular concentrations[1].
HDAC6-IN-75 (compound 3a) (34.0 μM HOG, 16.2 μM T98G, 50 μM U87MG, 28.9 μM U251MG; 24 h) induces DNA fragmentation (SubG1 accumulation) in T98G and U251MG cells, and modulates cell cycle progression by shifting populations from G0/G1 to G2/M in U87MG, U251MG, and HOG cells after 24 h of treatment[1].
HDAC6-IN-75 (compound 3a) (34.0 μM HOG, 16.2 μM T98G, 50 μM U87MG, 28.9 μM U251MG; 24 h) reduces viability and induces late apoptosis and necrosis in HOG and U251MG glioma cells after 24 h of treatment[1].
HDAC6-IN-75 (compound 3a) (0.0032-50 μM; 72 h) exhibits cytotoxicity against glioblastoma stem cells, with an IC50 of 3.725 μM in proneural GSC23 cells and 12.90 μM in mesenchymal GG16 cells after 72 h of treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Glioma cell lines HOG, T98G, U87MG, U251MG
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Concentration:34.0 μM (HOG); 16.2 μM (T98G); 50 μM (U87MG); 28.9 μM (U251MG)
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Incubation Time:24 h
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Result:Significantly promoted accumulation of acetyl-α-tubulin in HOG, T98G, U87MG, and U251MG cells.
Significantly promoted accumulation of acetyl-histone H3 in HOG, T98G, and U251MG cells, with activity comparable to vorinostat in these cell lines.
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Cell Line:Glioma cell lines HOG, T98G, U87MG, U251MG
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Concentration:34.0 μM (HOG); 16.2 μM (T98G); 50 μM (U87MG); 28.9 μM (U251MG)
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Incubation Time:24 h
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Result:Increased the percentage of cells in the SubG1 phase in T98G and U251MG cells.
Decreased the percentage of cells in the G0/G1 phase and increased the percentage in the G2/M phase in U87MG and U251MG cells.
Decreased the percentage of cells in the G0/G1 phase in HOG cells.
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Cell Line:Glioma cell lines HOG, T98G, U87MG, U251MG
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Concentration:34.0 μM (HOG); 16.2 μM (T98G); 50 μM (U87MG); 28.9 μM (U251MG)
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Incubation Time:24 h
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Result:Decreased cell viability in all four glioma cell lines.
Increased the percentage of late apoptotic and necrotic cells in HOG and U251MG cells.
Chemical Information
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CAS No. 400078-78-6
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Appearance Solid
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Masse moléculaire 292.31
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Formule C13H12N2O4S
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Color White to off-white
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SMILES
O=C(NO)C1=CC=C(NS(=O)(C2=CC=CC=C2)=O)C=C1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (342.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4210 mL | 17.1051 mL | 34.2103 mL | 85.5256 mL |
| 5 mM | 0.6842 mL | 3.4210 mL | 6.8421 mL | 17.1051 mL | |
| 10 mM | 0.3421 mL | 1.7105 mL | 3.4210 mL | 8.5526 mL | |
| 15 mM | 0.2281 mL | 1.1403 mL | 2.2807 mL | 5.7017 mL | |
| 20 mM | 0.1711 mL | 0.8553 mL | 1.7105 mL | 4.2763 mL | |
| 25 mM | 0.1368 mL | 0.6842 mL | 1.3684 mL | 3.4210 mL | |
| 30 mM | 0.1140 mL | 0.5702 mL | 1.1403 mL | 2.8509 mL | |
| 40 mM | 0.0855 mL | 0.4276 mL | 0.8553 mL | 2.1381 mL | |
| 50 mM | 0.0684 mL | 0.3421 mL | 0.6842 mL | 1.7105 mL | |
| 60 mM | 0.0570 mL | 0.2851 mL | 0.5702 mL | 1.4254 mL | |
| 80 mM | 0.0428 mL | 0.2138 mL | 0.4276 mL | 1.0691 mL | |
| 100 mM | 0.0342 mL | 0.1711 mL | 0.3421 mL | 0.8553 mL |
HDAC6-IN-75 Related Classifications
HY-181639 Related Classifications
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