Neotame
Based on 1 Customer Validation
Neotame is a derivative of Aspartame (HY-B0361) and is a flavor enhancer and low-caloric, non-nutritive, high-intensity artificial sweetener that is 7000-13,000 times sweeter than sugar. Neotame causes intestinal epithelial cell death at high concentrations. Neotame induces Apoptosis of Caco-2 cells.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 165450-17-9
- Formula: C20H30N2O5
- Molecular Weight:378.46
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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GPCR/G Protein Compound Library
HY-L006
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FDA-Approved Drug Library
HY-L022
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Anti-Cancer Compound Library
HY-L025
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Drug Repurposing Compound Library
HY-L035
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Orally Active Compound Library
HY-L061
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FDA Approved & Pharmacopeial Drug Library
HY-L066
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Food Additive Library
HY-L093
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Inactive Ingredient Library
HY-L096
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FDA-Approved Anticancer Drug Library
HY-L122
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Human Metabolite Library
HY-L123
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Off-patent Drug Library
HY-L141
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Drug Repurposing Compound Library Plus
HY-L035P
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FDA-Approved Drug Library Plus
HY-L022P
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FDA-Approved Drug Library Mini
HY-L022M
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
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Mass Spectrometry Human Metabolite Library
HY-L215
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Lactylation Compound Library
HY-L249
Biological Activity
Neotame (100 μM and higher, 6-24 h) causes intestinal epithelial cell death at high concentrations and leak across the epithelial monolayer at lower concentrations (1-100 μM)[3].
Neotame (0-1000 μM and higher, 6-24 h)-induced damage to the intestinal epithelium in vitro, both barrier disruption and cell death via apoptosis, is mediated by the sweet taste receptor, T1R3[3] .
Neotame (0-100 μM, 24 h) reduces Claudin 3 expression observed at Caco-2 cell surface[3] .
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Caco-2 cells
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Concentration:0, 0.01, 0.1, 1, 10, 100, 1000, 10000 μM
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Incubation Time:24 h
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Result:Showed very little cell viability at high concentrations.
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Cell Line:Caco-2 cells
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Concentration:0, 0.01, 0.1, 1, 10, 100, 1000, 10000 μM
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Incubation Time:6 h
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Result:Increased cell death from 100 μM and higher.
Increased significant apoptosis of Caco-2 cells from 10 μM and higher.
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Cell Line:Caco-2 cells
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Concentration:0, 10, 100 μM
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Incubation Time:24 h
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Result:Reduced Claudin 3 expression observed at Caco-2 cell surface.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ten male CD-1 mice around 7 weeks old[1].
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Dosage:0.75 mg/kg
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Administration:p.o., daily, 4 weeks
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Result:Induced lower alpha-diversity of gut microbiome than the control group.
Enriched phylum Bacteroidetes, while decreased Firmicutes.
Induced multiple components of family Lachnospiraceae and family Ruminococcaceae lower than controls, such as Blautia, Dorea, Oscillospira and Ruminococcus.
Enriched amino acid metabolism, LPS biosynthesis, antibiotics biosynthesis and folate biosynthesis pathways.
Induced lower fatty acid metabolism, carbohydrate metabolism, lipid metabolism and ABC transporters than in controls.
Decreased most of the altered metabolites, such as malic acid, mannose-6-phosphate, 5-aminovaleric acid and glyceric acid.
Decreased most of the identified lipids and fatty acids, including 1,3-dipalmitate, 1-monopalmitin, linoleic acid and stearic acid.
Increased the concentrations of cholesterol, campesterol and stigmastanol in the fecal samples.
Chemical Information
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CAS No. 165450-17-9
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Appearance Solid
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Molecular Weight 378.46
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Formula C20H30N2O5
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Color White to off-white
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SMILES
COC([C@H](CC1=CC=CC=C1)NC([C@H](CC(O)=O)NCCC(C)(C)C)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (264.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Liang Chi, et al. Effects of the Artificial Sweetener Neotame on the Gut Microbiome and Fecal Metabolites in Mice. Molecules. 2018 Feb 9;23(2):367. [Content Brief]
[2]. Anuradha Kumari, et al. Stability of Aspartame and Neotame in Pasteurized and In-Bottle Sterilized Flavoured Milk. Food Chem. 2016 Apr 1;196:533-8. [Content Brief]
[3]. Shil A, et al. The artificial sweetener neotame negatively regulates the intestinal epithelium directly through T1R3-signaling and indirectly through pathogenic changes to model gut bacteria. Front Nutr. 2024 Apr 24;11:1366409. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6423 mL | 13.2114 mL | 26.4229 mL | 66.0572 mL |
| 5 mM | 0.5285 mL | 2.6423 mL | 5.2846 mL | 13.2114 mL | |
| 10 mM | 0.2642 mL | 1.3211 mL | 2.6423 mL | 6.6057 mL | |
| 15 mM | 0.1762 mL | 0.8808 mL | 1.7615 mL | 4.4038 mL | |
| 20 mM | 0.1321 mL | 0.6606 mL | 1.3211 mL | 3.3029 mL | |
| 25 mM | 0.1057 mL | 0.5285 mL | 1.0569 mL | 2.6423 mL | |
| 30 mM | 0.0881 mL | 0.4404 mL | 0.8808 mL | 2.2019 mL | |
| 40 mM | 0.0661 mL | 0.3303 mL | 0.6606 mL | 1.6514 mL | |
| 50 mM | 0.0528 mL | 0.2642 mL | 0.5285 mL | 1.3211 mL | |
| 60 mM | 0.0440 mL | 0.2202 mL | 0.4404 mL | 1.1010 mL | |
| 80 mM | 0.0330 mL | 0.1651 mL | 0.3303 mL | 0.8257 mL | |
| 100 mM | 0.0264 mL | 0.1321 mL | 0.2642 mL | 0.6606 mL |
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