Netropsin dihydrochloride

Name: Netropsin dihydrochloride
Brand: MedChemExpress
SKU: HY-N6800A
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6800A Purity: 99.35%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Netropsin dihydrochloride is a small-molecule MGB (minor-groove binder), inhibits the catalytic activity of isolated topoisomerase and interferes with the stabilization of the cleavable complexes of topoisomerase II and I in nuclei. Netropsin dihydrochloride exhibits antiviral activity against the vaccinia virus.

For research use only. We do not sell to patients.

Netropsin dihydrochloride Chemical Structure

CAS No. : 18133-22-7

Size	Price	Stock	Quantity
5 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Netropsin dihydrochloride is a small-molecule MGB (minor-groove binder), inhibits the catalytic activity of isolated topoisomerase and interferes with the stabilization of the cleavable complexes of topoisomerase II and I in nuclei^[1]. Netropsin dihydrochloride exhibits antiviral activity against the vaccinia virus^[4].

In Vitro

Netropsin dihydrochloride has potential antibiotic and antiviral properties by binding to dsDNA in a non-intercalative manner, it improves survival from murine endotoxaemia by attenuating NOS2 induction through interference with HMGA1 DNA binding to the core NOS2 promoter^[2].
Netropsin dihydrochloride in solutions of DNA has radioprotective ability against radiation-induced damage due to its high binding affinity and high structural stabilization, hydroxyl radical (OH*) generated by ionizing radiation cannot radiolyse the netropsin-DNA complex^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

503.39

Formula

C₁₈H₂₈Cl₂N₁₀O₃

CAS No.

18133-22-7

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(C1=CC(NC(CNC(N)=N)=O)=CN1C)NC2=CN(C)C(C(NCCC(N)=N)=O)=C2.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (248.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 20 mg/mL (39.73 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9865 mL	9.9327 mL	19.8653 mL
5 mM	0.3973 mL	1.9865 mL	3.9731 mL
10 mM	0.1987 mL	0.9933 mL	1.9865 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.35%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (245 KB) HNMR (281 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Beerman TA et al. Netropsin and bis-netropsin analogs as inhibitors of the catalytic activity of mammalian DNA topoisomerase II and topoisomerase cleavable complexes. Biochim Biophys Acta. 1991 Aug 27;1090(1):52-60. [Content Brief]

[2]. Grant MA, et al. Netropsin improves survival from endotoxaemia by disrupting HMGA1 binding to the NOS2 promoter. Biochem J. 2009 Feb 15;418(1):103-12. [Content Brief]

[3]. Mishra K et al. Netropsin, a minor groove binding ligand: a potential radioprotective agent. Radiat Res. 2009 Dec;172(6):698-705. [Content Brief]

[4]. Smee DF, et, al. A review of compounds exhibiting anti-orthopoxvirus activity in animal models. Antiviral Res. 2003 Jan;57(1-2):41-52. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.9865 mL	9.9327 mL	19.8653 mL	49.6633 mL
	5 mM	0.3973 mL	1.9865 mL	3.9731 mL	9.9327 mL
	10 mM	0.1987 mL	0.9933 mL	1.9865 mL	4.9663 mL
	15 mM	0.1324 mL	0.6622 mL	1.3244 mL	3.3109 mL
	20 mM	0.0993 mL	0.4966 mL	0.9933 mL	2.4832 mL
	25 mM	0.0795 mL	0.3973 mL	0.7946 mL	1.9865 mL
	30 mM	0.0662 mL	0.3311 mL	0.6622 mL	1.6554 mL
DMSO	40 mM	0.0497 mL	0.2483 mL	0.4966 mL	1.2416 mL
	50 mM	0.0397 mL	0.1987 mL	0.3973 mL	0.9933 mL
	60 mM	0.0331 mL	0.1655 mL	0.3311 mL	0.8277 mL
	80 mM	0.0248 mL	0.1242 mL	0.2483 mL	0.6208 mL
	100 mM	0.0199 mL	0.0993 mL	0.1987 mL	0.4966 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.