Neutral Red Staining Solution (for Live Cells)

Neutral Red Staining Solution (for Live Cells) (1:100,000; 1-6 h) enables reliable live-dead differentiation of diverse estuarine zooplankton taxa, with staining times ranging from 1 hour for calanoid copepods and polychaete larvae to 6 hours for barnacle nauplii and decapod zoeas, where live organisms stain deep red and dead organisms stain white to pink^[1].
Neutral Red Staining Solution (for Live Cells) (1:50,000; 1 h) enables reliable live-dead differentiation of Biddulphia, Melosira, and Peridinium, where live cells exhibit ruby-red cytoplasm and dead cells have unstained cytoplasm^[1].
Neutral Red Staining Solution (for Live Cells) (1:200,000; 1 h) enables reliable live-dead differentiation of Eurytemora herdmani, polychaete larvae, calanoid copepods, and multiple other estuarine zooplankton taxa, where live organisms stain deep red and dead organisms stain white to pink^[1].
Neutral Red Staining Solution (for Live Cells) (1:1 dilution of 1 mg/mL stock with 1.8% NaCl; 1.5 h) produces absorbance at 550 nm that is linearly proportional to cell number in primary rat osteoblast-like cells, UMR-106, SaOS-2, and MG-63 cells, with correlation coefficients >0.99^[2].
The Neutral Red Staining Solution (for Live Cells) (1.5 h; 1-5 days) detects dose-dependent growth of primary rat osteoblast-like cells (ROB) in the presence of 10% FCS, with linear increases in OD₅₅₀ between days 2 and 4, and no increase in serum-free conditions^[2].
Neutral Red Staining Solution (for Live Cells) (1.5 h; 4 h) produces absorbance at 550 nm that is linearly proportional to cell number in nonadherent CTLL-2 and B-9 cells, with correlation coefficients >0.99, and results comparable to the [³H]thymidine incorporation assay for measuring cytokine-induced cell proliferation^[2].
The Neutral Red Staining Solution (for Live Cells) (1.5 h; 96 h) provides a reproducible and sensitive measure of rhIL-6-induced proliferation of nonadherent B-9 cells, with dose-dependent increases in OD₅₅₀ across three independent experiments^[2].
The Neutral Red Staining Solution (for Live Cells) (0.03-3 mg/mL; 2 h-overnight) accurately detects cell viability in ES-2, A2780, S2VP10, and Panc1 cells across extracellular pH values 7.4, 6.8, and 6.6, with significant (p < 0.05) correlations to apoptotic and autophagic marker levels, enabling reliable evaluation of chemotherapeutic agent cytotoxicity under acidic tumor-like microenvironment conditions^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.