ODN 1585 sodium
Based on 1 Customer Validation
ODN 1585 sodium is a potent inducer of IFN and TNFα production. ODN 1585 sodium is a potent stimulator of NK (natural killer) function. ODN 1585 sodium increases CD8+ T-cell function, including the CD8+ T cell-mediated production of IFN-γ. ODN 1585 sodium induces regression of established melanomas in mice. ODN 1585 sodium can confer complete protection against malaria in mice. ODN 1585 sodium can be used for acute myelogenous leukemia (AML) and malaria research. ODN 1585 sodium can be used as a vaccine adjuvant.
For research use only. We do not sell to patients.
- Purity: 92.48%
- Molecular Weight:6430 (free acid)
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
ODN 1585 (3 μg/mL, 48 h) sodium induces PBMC producing IFN-α in the nanogram range[3].
ODN 1585 sodium increases the percentage of CD69+ (early marker of activation) NK cells within 24 h (26%)[3].
CpG ODN (0.6μg/mL, 18 h) sodium stimulates NK cell-mediated lysis of K562 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
ODN 1585 (100 μg/dose, IP, twice weekly) sodium is determined to be optimal for the induction of antitumor responses in several systems involving comparisons of 30, 100, and 300 μg/injection[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c ByJ mice (4- to 8-week-old, female, 6-18 mice in each group)[1]
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Dosage:50, 100, 200, or 500 μg (in 50 μL of saline)
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Administration:Injection into the tibialis anterior muscle, single (at 7, 2, or 1 day(s) prior to sporozoite infection, on the day of infection, and/or at 1 day postinfection)
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Result:Protected 20 to 90% of mice from infection when the ODN 1585 was administered around the time of sporozoite challenge with doses of 50 to 500 μg. The highest level of protection (90%) resulted from administration of 200 μg of CpG ODN 1585 the day before challenge or 100 μg of CpG ODN 1585 on the day before and the day of challenge.
Chemical Information
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Appearance Solid
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Molecular Weight 6430 (free acid)
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Color White to off-white
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SMILES
[DNA, d(G-sp-G-G-G-T-C-A-A-C-G-T-T-G-A-G-G-sp-G-sp-G-sp-G-sp-G), sodium salt]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2242 KB)
References
[1]. Gramzinski RA, et al. Interleukin-12- and gamma interferon-dependent protection against malaria conferred by CpG oligodeoxynucleotide in mice. Infect Immun. 2001 Mar;69(3):1643-9. [Content Brief]
[2]. Blazar BR, et al. Synthetic unmethylated cytosine-phosphate-guanosine oligodeoxynucleotides are potent stimulators of antileukemia responses in naive and bone marrow transplant recipients. Blood. 2001 Aug 15;98(4):1217-25. [Content Brief]
[3]. Krug A, et al. Identification of CpG oligonucleotide sequences with high induction of IFN-alpha/beta in plasmacytoid dendritic cells. Eur J Immunol. 2001 Jul;31(7):2154-63. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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