PD-1/PD-L1-IN-66 

PD-1/PD-L1-IN-66 is a PD-1/PD-L1 immune checkpoint inhibitor with an IC₅₀ of 3 nM for blocking the PD-1/PD-L1 interaction. PD-1/PD-L1-IN-66 binds to the interface of PD-L1 dimers with a K_d value of 0.75 nM. PD-1/PD-L1-IN-66 can reactivate the proliferation of cytotoxic T cells. PD-L1 ligand-2 is applicable for cancer research^[1].

PD-1/PD-L1-IN-66 (Compound 12) binds to purified PD-L1 protein with a K_d of 7 nM and to the PD-L1 dimer interface with a K_d of 0.75 nM^[1].
PD-1/PD-L1-IN-66 reactivates proliferation of hPD-L1-exposed CTLL-2 cytotoxic T-cells with an IC₅₀ of 124 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

339.60

C₁₅H₉Cl₃N₂O

O=C1CC(C2=CC=C(C=C2)Cl)=NN1C3=C(C=C(C=C3)Cl)Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Klupsch F, et al. Chlorophenylpyrazolone derivatives as novel PD-L1-targeted inhibitors of the PD-1/PD-L1 immune checkpoint. Bioorg Med Chem Lett. 2026 May 25;139:130694.