Evaporative light scattering detection of pyrrolizidine alkaloids

  • Phytochem Anal. 2004 Jan-Feb;15(1):36-9. doi: 10.1002/pca.715.
Brian T Schaneberg  1 ,  Russell J Molyneux ,  Ikhlas A Khan
Affiliations
  • 1. National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA.
PMID: 14979525 DOI: 10.1002/pca.715
Abstract

A reverse-phase high-performance liquid chromatography method utilizing evaporative light scattering detection (ELSD) has been developed for the simultaneous detection of hepatotoxic Pyrrolizidine Alkaloids with and without chromophores, namely, riddelliine, riddelliine N-oxide, senecionine, senecionine N-oxide, seneciphylline, retrorsine, integerrimine, lasiocarpine and heliotrine. Pyrrolizidine Alkaloids were detected in five plant extracts (Senecio spartioides, S. douglasii var. longilobus, S. jacobaea, S. intergerrimus var. exaltatus and Symphytum officinale). The detection of heliotrine (which does not contain a chromophore) was much improved by ELSD compared with photodiode array detection.

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