Cytotoxicity of a new tirucallane derivative isolated from Stereospermum acuminatissimum K. Schum stem bark

  • Nat Prod Res. 2021 Nov;35(22):4417-4422. doi: 10.1080/14786419.2020.1723085.
Bosco Peron Leutcha  1  2 ,  Denis Kehdinga Sema  1  2 ,  Jean Paul Dzoyem  3  4 ,  Godfred Aponglen Ayimele  5 ,  Kennedy D Nyongbela  5 ,  Florence Delie  4 ,  Éric Alléman  4 ,  Norbert Sewald  6 ,  Alain Meli Lannang  2  6
Affiliations
  • 1. Department of Chemistry, Faculty of Science, University of Maroua, Maroua, Cameroon.
  • 2. Department of Chemistry, Higher Teachers' Training College, University of Maroua, Maroua, Cameroon.
  • 3. Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
  • 4. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.
  • 5. Department of Chemistry, University of Buea, Buea, Cameroon.
  • 6. Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Bielefeld, Germany.
PMID: 32011174 DOI: 10.1080/14786419.2020.1723085
Abstract

One new tirucallan derivative, leutcharic acid (1) was isolated from Stereospermum acuminatissimum stem bark together with the known compounds 3-oxo-22-hydroxyhopane (2), 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic acid (3), 3-oxotirucalla-7,24-dien-21-oic acid (7), lupeol (4), β-sitosterol (5) and stigmasterol (6). The structures of the isolated compounds were elucidated using 1 D and 2 D NMR spectroscopy in combination with literature data. To the best of our knowledge, this is the first report on the cytotoxic properties' constituents of S. acuminatissimum. Cytotoxicity of compounds 1 and 2 was assessed in vitro with the WST-1 assay on human lung adenocarcinoma A549 and THP-1 human monocytic leukaemia cell lines. Both compounds showed antiproliferative activity on the Cancer cells. Compound 2 was more active against THP-1 with an IC50 value of 26.83 µM. The sensitivity of THP-1 cells to compounds 1 and 2 indicated that these compounds might be potential leads for Anticancer agent development against leukaemia.

Keywords
Stereospermum acuminatissimum; Triterpene; antiproliferative activity; cytotoxicity.
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