Isolation, Identification, and Anti-Inflammatory Activity of Polysaccharides of Typha angustifolia

  • Biomacromolecules. 2021 Jun 14;22(6):2451-2459. doi: 10.1021/acs.biomac.1c00235.
Huan Wei  1 ,  Yuqi Shi  2 ,  Zhixiang Yuan  2 ,  Zhinan Huang  1 ,  Fuhong Cai  1 ,  Jingfeng Zhu  1 ,  Wanwan Zhang  1 ,  Jia Li  1 ,  Qingping Xiong  1 ,  Yunpeng Wang  1 ,  Xiaoli Wang  1
Affiliations
  • 1. Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, College of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China.
  • 2. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
PMID: 34024108 DOI: 10.1021/acs.biomac.1c00235
Abstract

The present study aimed to purify, structurally characterize, and evaluate the anti-inflammatory activity of the polysaccharide extracted from Typha angustifolia. Two purified Polysaccharides (PTA-1 and PTA-2) were obtained via DEAE-52 cellulose chromatography. Their structural characterizations and antioxidant activity were in vitro analyzed. To evaluate the anti-inflammatory activity of PTA-2, the levels of inflammatory cytokines, intracellular ROS production, and the inhibitory effects of the transcriptional activation of the nuclear factor kappa B (NF-κB) signaling pathway were determined. PTA-1 comprises glucose (100%) with α-(1 → 3) glycosidic bonds, and PTA-2 comprises glucose (66.7%) and rhamnose (33.3%) formed by β-(1 → 3) glycosidic bonds. PTA-1 and PTA-2 showed strong antioxidant activity in vitro. Moreover, PTA-2 intervention (50, 100, and 200 μg/mL) suppressed the production of inflammatory cytokines, the activation of NF-κB signaling, and Reactive Oxygen Species production significantly. The results identified PTA-2 as a natural product that could be applied in anti-inflammatory drugs.

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