New dihydro-β-agarofuran sesquiterpenoids from Tripterygium wilfordii and their anti-inflammatory activity

  • Bioorg Chem. 2021 Sep:114:105140. doi: 10.1016/j.bioorg.2021.105140.
Bixuan Cao  1 ,  Peipei Guan  1 ,  Minglei Li  1 ,  Bo Liu  1 ,  Yingying Xu  1 ,  Zengguang Zhang  1 ,  Li Han  2 ,  Xueshi Huang  3
Affiliations
  • 1. Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, People's Republic of China.
  • 2. Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, People's Republic of China. Electronic address: [email protected].
  • 3. Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, People's Republic of China. Electronic address: [email protected].
PMID: 34229200 DOI: 10.1016/j.bioorg.2021.105140
Abstract

Twenty seven dihydro-β-agarofuran sesquiterpenoids, including fifteen new congeners, wilforsinines I-W (1-9, 12-13, 24-27), and twelve known compounds were isolated from the dried root of Tripterygium wilfordii. The structures of the new sesquiterpenoids, wilforsinines I-W, were elucidated by extensive spectroscopic data analysis. The anti-inflammatory activity of isolates 1-27 were evaluated by examining their inhibitory effects on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophage cells. Among them, wilforsinine K (3) and angulatin M (16) exerted optimal inhibitory effects on the production of NO in LPS-induced RAW 264.7 cells. Moreover, Western blot results revealed that their anti-inflammatory activities were correlated with the suppression of the expression of nitric oxide synthase (iNOS) and down-regulation of the level of NF-κB p65 phosphorylation.

Keywords
Anti-inflammation; Dihydro-β-agarofuran sesquiterpenoids; NF-κB; Tripterygium wilfordii; Wilforsinines.
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