A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation

  • Nat Commun. 2024 Jun 14;15(1):5107. doi: 10.1038/s41467-024-49102-6.
Li Lin Xu  1  2 ,  Meng Qi Cui  1  2 ,  Chen Xu  1  2 ,  Miao Jing Zhang  1 ,  Gui Xin Li  3 ,  Ji Ming Xu  1 ,  Xiao Dan Wu  4 ,  Chuan Zao Mao  1 ,  Wo Na Ding  5 ,  Moussa Benhamed  6 ,  Zhong Jie Ding  7 ,  Shao Jian Zheng  8  9
Affiliations
  • 1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China.
  • 2. Guangdong Laboratory for Lingnan Modern Agriculture, College of Natural Resources and Environment, South China Agricultural University, 5100642, Guangzhou, China.
  • 3. College of Agronomy and Biotechnology, Zhejiang University, 310058, Hangzhou, China.
  • 4. Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, 310058, Hangzhou, China.
  • 5. Ningbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, 315300, Ningbo, China.
  • 6. Université Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 10 91405, Orsay, France.
  • 7. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China. [email protected].
  • 8. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 310058, Hangzhou, China. [email protected].
  • 9. Guangdong Laboratory for Lingnan Modern Agriculture, College of Natural Resources and Environment, South China Agricultural University, 5100642, Guangzhou, China. [email protected].
PMID: 38877001 DOI: 10.1038/s41467-024-49102-6
Abstract

Inositol hexaphosphate (InsP6) is the major storage form of phosphorus in seeds. Reducing seed InsP6 content is a breeding objective in agriculture, as InsP6 negatively impacts animal nutrition and the environment. Nevertheless, how InsP6 accumulation is regulated remains largely unknown. Here, we identify a clade of receptor-like cytoplasmic kinases (RLCKs), named Inositol Polyphosphate-related Cytoplasmic Kinases 1-6 (IPCK1-IPCK6), deeply involved in InsP6 accumulation. The InsP6 concentration is dramatically reduced in seeds of ipck quadruple (T-4m/C-4m) and quintuple (C-5m) mutants, accompanied with the obviously increase of phosphate (Pi) concentration. The plasma membrane-localized IPCKs recruit IPK1 involved in InsP6 synthesis, and facilitate its binding and activity via phosphorylation of GRF 14-3-3 proteins. IPCKs also recruit IPK2s and PI-PLCs required for InsP4/InsP5 and InsP3 biosynthesis respectively, to form a potential IPCK-GRF-PLC-IPK2-IPK1 complex. Our findings therefore uncover a regulatory mechanism of InsP6 accumulation governed by IPCKs, shedding light on the mechanisms of InsP biosynthesis in eukaryotes.

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