30S Ribosomal Protein S4/RPS4 Protein, E.coli (His)
30S Ribosomal Protein S4, E.coli (E. coli, His) is a recombinant E. coli 30S Ribosomal Protein S4 expressed in E. coli with a His tag at the N-terminus. Ribosomal protein S4 plays a central role in the biogenesis of the small ribosomal subunit.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
30S Ribosomal Protein S4, E.coli (E. coli, His) is a recombinant E. coli 30S Ribosomal Protein S4 expressed in E. coli with a His tag at the N-terminus. Ribosomal protein S4 plays a central role in the biogenesis of the small ribosomal subunit[1].
Background
Primary ribosomal protein S4 is essential for 30S ribosome biogenesis in eubacteria, because it nucleates subunit assembly and helps coordinate assembly with the synthesis of its rRNA and protein components[1].
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-His
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Accession
P0A7V8 (A2-K206)
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Gene ID947793 [NCBI]
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Molecular Construction
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N-term
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His
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RS4_ECOLI (A2-K206)
Accession # P0A7V8 -
C-term
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Protein Length
Full Length
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Synonyms
Small ribosomal subunit protein uS4; 30S ribosomal protein S4; rpsD; ramA; b3296; JW3258
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AA Sequence
ARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK
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Predicted Molecular Mass
27.3 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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