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  5. 30S Ribosomal Protein S4/RPS4 Protein, E.coli (His)

30S Ribosomal Protein S4/RPS4 Protein, E.coli (His)

Cat. No.: HY-P71689
Handling Instructions

30S Ribosomal Protein S4, E.coli (E. coli, His) is a recombinant E. coli 30S Ribosomal Protein S4 expressed in E. coli with a His tag at the N-terminus. Ribosomal protein S4 plays a central role in the biogenesis of the small ribosomal subunit.

For research use only. We do not sell to patients.

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Description

30S Ribosomal Protein S4, E.coli (E. coli, His) is a recombinant E. coli 30S Ribosomal Protein S4 expressed in E. coli with a His tag at the N-terminus. Ribosomal protein S4 plays a central role in the biogenesis of the small ribosomal subunit[1].
Background

Primary ribosomal protein S4 is essential for 30S ribosome biogenesis in eubacteria, because it nucleates subunit assembly and helps coordinate assembly with the synthesis of its rRNA and protein components[1].
Species

E.coli
Source

E. coli
Tag

N-His
Accession

P0A7V8 (2A-206K)
Gene ID

58463221  [NCBI]
Molecular Construction
N-term
His
RS4_ECOLI (2A-206K)
Accession # P0A7V8
C-term
Synonyms
rpsD; ramA; b3296; JW3258; 30S ribosomal protein S4; Small ribosomal subunit protein uS4
AA Sequence

ARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK

Molecular Weight

Approximately 27.3 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)
References

30S Ribosomal Protein S4/RPS4 Protein, E.coli (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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30S Ribosomal Protein S4/RPS4 Protein, E.coli (His)
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