ABCA1 Protein, Human (HEK293, His, StrepII, FLAG)
Based on 1 Customer Validation
The ABCA1 protein catalyzes the translocation of specific phospholipids from the cytoplasm to the extracellular/luminal membrane leaflet in response to ATP hydrolysis. It achieves phospholipid translocation by forcing phospholipids through its channels and loops into elongated hydrophobic tunnels in its extracellular domain. ABCA1 Protein, Human (HEK293, His, StrepⅡ, FLAG) is the recombinant human-derived ABCA1 protein, expressed by HEK293 , with N-StrepⅡ, C-Flag, N-8*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ABCA1 protein catalyzes the translocation of specific phospholipids from the cytoplasm to the extracellular/luminal membrane leaflet in response to ATP hydrolysis. It achieves phospholipid translocation by forcing phospholipids through its channels and loops into elongated hydrophobic tunnels in its extracellular domain. ABCA1 Protein, Human (HEK293, His, StrepⅡ, FLAG) is the recombinant human-derived ABCA1 protein, expressed by HEK293 , with N-StrepⅡ, C-Flag, N-8*His labeled tag.
Background
ABCA1 protein functions as a crucial catalyst in the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of the membrane, a process driven by ATP hydrolysis. This activity is integral to the transfer of phospholipids to apolipoproteins, leading to the formation of nascent high-density lipoproteins (HDLs). ABCA1 exhibits a preference for transporting phosphatidylcholine over phosphatidylserine and may play a similar role in the efflux of intracellular cholesterol to apolipoproteins, contributing to the generation of nascent HDLs. The mechanism involves the translocation of phospholipids from the outer face of the plasma membrane, forcing them through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain. This intricate process is essential for the regulation of lipid homeostasis and the maintenance of cellular and systemic lipid balance.
Technical Parameters
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Species Human
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Source HEK293
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Tag N-StrepⅡ;N-8*His;C-Flag
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Accession
O95477 (A2-V2261)
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Gene ID19
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Molecular Construction
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N-term
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8*His-StrepⅡ
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ABCA1 (A2-V2261)
Accession # O95477 -
Flag
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C-term
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Protein Length
Full Length
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Synonyms
ABCA1; ATP-Binding Cassette Transporter A1; Prev. ABC1; ATP Binding Cassette Transporter 1; Prev. HDLDT1; P-Type Phospholipid Transporter; TGD; Alternative Protein ABCA1; ATP-Binding Cassette, Sub-Family A (ABC1), Member 1; ATP-Binding Cassette 1; ATP-Bin
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AA Sequence
ACWPQLRLLLWKNLTFRRRQTCQLLLEVAWPLFIFLILISVRLSYPPYEQHECHFPNKAMPSAGTLPWVQGIICNANNPCFRYPTPGEAPGVVGNFNKSIVARLFSDARRLLLYSQKDTSMKDMRKVLRTLQQIKKSSSNLKLQDFLVDNETFSGFLYHNLSLPKSTVDKMLRADVILHKVFLQGYQLHLTSLCNGSKSEEMIQLGDQEVSELCGLPREKLAAAERVLRSNMDILKPILRTLNSTSPFPSKELAEATKTLLHSLGTLAQELFSMRSWSDMRQEVMFLTNVNSSSSSTQIYQAVSRIVCGHPEGGGLKIKSLNWYEDNNYKALFGGNGTEEDAETFYDNSTTPYCNDLMKNLESSPLSRIIWKALKPLLVGKILYTPDTPATRQVMAEVNKTFQELAVFHDLEGMWEELSPKIWTFMENSQEMDLVRMLLDSRDNDHFWEQQLDGLDWTAQDIVAFLAKHPEDVQSSNGSVYTWREAFNETNQAIRTISRFMECVNLNKLEPIATEVWLINKSMELLDERKFWAGIVFTGITPGSIELPHHVKYKIRMDIDNVERTNKIKDGYWDPGPRADPFEDMRYVWGGFAYLQDVVEQAIIRVLTGTEKKTGVYMQQMPYPCYVDDIFLRVMSRSMPLFMTLAWIYSVAVIIKGIVYEKEARLKETMRIMGLDNSILWFSWFISSLIPLLVSAGLLVVILKLGNLLPYSDPSVVFVFLSVFAVVTILQCFLISTLFSRANLAAACGGIIYFTLYLPYVLCVAWQDYVGFTLKIFASLLSPVAFGFGCEYFALFEEQGIGVQWDNLFESPVEEDGFNLTTSVSMMLFDTFLYGVMTWYIEAVFPGQYGIPRPWYFPCTKSYWFGEESDEKSHPGSNQKRISEICMEEEPTHLKLGVSIQNLVKVYRDGMKVAVDGLALNFYEGQITSFLGHNGAGKTTTMSILTGLFPPTSGTAYILGKDIRSEMSTIRQNLGVCPQHNVLFDMLTVEEHIWFYARLKGLSEKHVKAEMEQMALDVGLPSSKLKSKTSQLSGGMQRKLSVALAFVGGSKVVILDEPTAGVDPYSRRGIWELLLKYRQGRTIILSTHHMDEADVLGDRIAIISHGKLCCVGSSLFLKNQLGTGYYLTLVKKDVESSLSSCRNSSSTVSYLKKEDSVSQSSSDAGLGSDHESDTLTIDVSAISNLIRKHVSEARLVEDIGHELTYVLPYEAAKEGAFVELFHEIDDRLSDLGISSYGISETTLEEIFLKVAEESGVDAETSDGTLPARRNRRAFGDKQSCLRPFTEDDAADPNDSDIDPESRETDLLSGMDGKGSYQVKGWKLTQQQFVALLWKRLLIARRSRKGFFAQIVLPAVFVCIALVFSLIVPPFGKYPSLELQPWMYNEQYTFVSNDAPEDTGTLELLNALTKDPGFGTRCMEGNPIPDTPCQAGEEEWTTAPVPQTIMDLFQNGNWTMQNPSPACQCSSDKIKKMLPVCPPGAGGLPPPQRKQNTADILQDLTGRNISDYLVKTYVQIIAKSLKNKIWVNEFRYGGFSLGVSNTQALPPSQEVNDAIKQMKKHLKLAKDSSADRFLNSLGRFMTGLDTKNNVKVWFNNKGWHAISSFLNVINNAILRANLQKGENPSHYGITAFNHPLNLTKQQLSEVALMTTSVDVLVSICVIFAMSFVPASFVVFLIQERVSKAKHLQFISGVKPVIYWLSNFVWDMCNYVVPATLVIIIFICFQQKSYVSSTNLPVLALLLLLYGWSITPLMYPASFVFKIPSTAYVVLTSVNLFIGINGSVATFVLELFTDNKLNNINDILKSVFLIFPHFCLGRGLI
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Molecular Weight
Approximately 258 KDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 20 mM HEPES pH 7.5, 150 mM NaCl, 2 mM DTT, cOmplete Protease Inhibitor Tablet, 0.005% (w/v) GDN.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (246 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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