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  4. ACAT2 Protein, Human (His, Myc)

ACAT2 Protein, Human (His, Myc)

Cat. No.: HY-P701332
COA Handling Instructions

ACAT2 Protein is essential in cholesterol biosynthesis. ACAT2 Protein, Human (His, Myc) is the recombinant human-derived ACAT2 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of ACAT2 Protein, Human (His, Myc) is 397 a.a., with molecular weight of 48.8 kDa.

For research use only. We do not sell to patients.

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20 μg $140 In-stock
50 μg $305 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

ACAT2 Protein is essential in cholesterol biosynthesis. ACAT2 Protein, Human (His, Myc) is the recombinant human-derived ACAT2 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of ACAT2 Protein, Human (His, Myc) is 397 a.a., with molecular weight of 48.8 kDa.

Background

ACAT2 Protein is a key player in the biosynthetic pathway of cholesterol.

Species

Human

Source

E. coli

Tag

N-10*His;C-Myc

Accession

Q9BWD1 (M1-E397)

Gene ID

39

Molecular Construction
N-term
10*His
ACAT2 (M1-E397)
Accession # Q9BWD1
Myc
C-term
Synonyms
Acetyl-CoA acetyltransferase cytosolic; Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase; ACTL; ACAT2
Molecular Weight

48.8 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ACAT2 Protein, Human (His, Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ACAT2 Protein, Human (His, Myc)
Cat. No.:
HY-P701332
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