ACBD6 Protein, Human (sf9, His)
ACBD6 protein selectively binds long-chain acyl-CoA, showing a significant preference for unsaturated C18:1-CoA. Unlike other variants such as unsaturated C20:4-CoA and saturated C16:0-CoA, ACBD6 does not bind to free fatty acids. ACBD6 Protein, Human (sf9, His) is the recombinant human-derived ACBD6 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ACBD6 protein selectively binds long-chain acyl-CoA, showing a significant preference for unsaturated C18:1-CoA. Unlike other variants such as unsaturated C20:4-CoA and saturated C16:0-CoA, ACBD6 does not bind to free fatty acids. ACBD6 Protein, Human (sf9, His) is the recombinant human-derived ACBD6 protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
The ACBD6 Protein plays a distinctive role in cellular metabolism by selectively binding long-chain acyl-coenzyme A molecules, demonstrating a notable preference for unsaturated C18:1-CoA over other variants such as unsaturated C20:4-CoA and saturated C16:0-CoA. Importantly, ACBD6 does not exhibit binding affinity for free fatty acids. Structurally, the protein exists as a monomer, suggesting its functional activity is associated with its individual unit rather than complex formations. This specificity in acyl-coenzyme A binding underscores the intricate molecular recognition mechanisms involved in lipid metabolism, highlighting ACBD6's role in cellular processes related to acyl-CoA interactions.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
Q9BR61 (M1-A282)
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Molecular Construction
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N-term
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His
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ACBD6 (M1-A282)
Accession # Q9BR61 -
C-term
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Protein Length
Full Length
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Synonyms
ACBD6; Acyl-Coenzyme A Binding Domain Containing 6; Acyl-CoA Binding Domain Containing 6; MGC2404; Acyl-CoA-Binding Domain-Containing Protein 6; NEDPM
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AA Sequence
MASSFLPAGAITGDSGGELSSGDDSGEVEFPHSPEIEETSCLAELFEKAAAHLQGLIQVASREQLLYLYARYKQVKVGNCNTPKPSFFDFEGKQKWEAWKALGDSSPSQAMQEYIAVVKKLDPGWNPQIPEKKGKEANTGFGGPVISSLYHEETIREEDKNIFDYCRENNIDHITKAIKSKNVDVNVKDEEGRALLHWACDRGHKELVTVLLQHRADINCQDNEGQTALHYASACEFLDIVELLLQSGADPTLRDQDGCLPEEVTGCKTVSLVLQRHTTGKA
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Predicted Molecular Mass
33.4 kDa
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Molecular Weight
Approximately 36 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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