ACKA Protein, Bacteria (His)
ACKA Protein, Bacteria (His) is the recombinant ACKA, expressed by E. coli, with His labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ACKA Protein, Bacteria (His) is the recombinant ACKA, expressed by E. coli, with His labeled tag.
Background
ACKA is an enzyme that catalyzes the formation of acetyl phosphate from acetate and ATP, as well as the reverse reaction. During anaerobic growth, it also participates in the synthesis of most ATP formed catabolically. It serves as the primary pathway for acetate production during the exponential phase.
Technical Parameters
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Species Others
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Source E. coli
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Tag His
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Accession
P0A6A3 (M1-A400)
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Gene ID946775
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Protein Length
Full Length
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Synonyms
Acetate kinase; EC:2.7.2.1; Acetokinase; ackA; ack; b2296; JW2293
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AA Sequence
MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
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Molecular Weight
Approximately 46 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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