ACTB Protein, Human (His)
Based on 1 Customer Validation
ACTB Protein, Human (His) is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Phe375) of Human ACTB fused with a His tag at the C-terminus.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
ACTB Protein, Human (His) is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Phe375) of Human ACTB fused with a His tag at the C-terminus[1].
ACTB encodes b-actin, an abundant cytoskeletal housekeeping protein. ACTB is the only gene common to all four deletions, leading us to hypothesize that ACTB haploinsufficiency leads to a distinct clinical syndrome[1].
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P60709 (D2-F375)
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Molecular Construction
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N-term
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ACTB (D2-F375)
Accession # P60709 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
rHuACTB, His; Actin cytoplasmic 1; ACTB
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AA Sequence
DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
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Predicted Molecular Mass
42.8 kDa
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Molecular Weight
Approximately 43 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
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≥ 90%, as determined by reducing SDS-PAGE.
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Product Properties
Solution
Supplied as a 0.22 μm filter solution of 10 mM Tris-HCl, 0.1% Triton X-100, 2 mM DTT, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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