ADAM9 Protein, Human (492a.a, HEK293, His)

ADAM9, a metalloprotease, cleaves and releases TEK, KDR, EPHB4, CD40, VCAM1, and CDH5, playing a pivotal role in tumorigenesis and angiogenesis. It regulates cell motility through integrin interactions, mediates cell-cell and cell-matrix interactions, and may act as an alpha-secretase for APP, suggesting involvement in neurobiology and neurodegenerative disorders. ADAM9 Protein, Human (492a.a, HEK293, His) is the recombinant human-derived ADAM9 protein, expressed by HEK293, with C-His labeled tag.

ADAM9, a metalloprotease, plays a pivotal role in tumorigenesis and angiogenesis by cleaving and releasing various molecules including TEK, KDR, EPHB4, CD40, VCAM1, and CDH5. Its multifaceted functions extend to mediating cell-cell and cell-matrix interactions, thereby regulating cell motility through interactions with integrins. Additionally, ADAM9 may function as an alpha-secretase for the amyloid precursor protein (APP), highlighting its involvement in processes relevant to neurobiology and neurodegenerative disorders.

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH₂. The specific activity is >5.0 pmol/min/μg.

Human

HEK293

C-His

Q13443-1 (A206-D697)

8754

Partial

AVLPQTRYVELFIVVDKERYDMMGRNQTAVREEMILLANYLDSMYIMLNIRIVLVGLEIWTNGNLINIVGGAGDVLGNFVQWREKFLITRRRHDSAQLVLKKGFGGTAGMAFVGTVCSRSHAGGINVFGQITVETFASIVAHELGHNLGMNHDDGRDCSCGAKSCIMNSGASGSRNFSSCSAEDFEKLTLNKGGNCLLNIPKPDEAYSAPSCGNKLVDAGEECDCGTPKECELDPCCEGSTCKLKSFAECAYGDCCKDCRFLPGGTLCRGKTSECDVPEYCNGSSQFCQPDVFIQNGYPCQNNKAYCYNGMCQYYDAQCQVIFGSKAKAAPKDCFIEVNSKGDRFGNCGFSGNEYKKCATGNALCGKLQCENVQEIPVFGIVPAIIQTPSRGTKCWGVDFQLGSDVPDPGMVNEGTKCGAGKICRNFQCVDASVLNYDCDVQKKCHGHGVCNSNKNCHCENGWAPPNCETKGYGGSVDSGPTYNEMNTALRD

Approximately 60-70 kD,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as 0.22 μm filtered solution of 50 mM Tris, 500 mM NaCL, 0.005% Brij-35, 20% Glycerol, pH 8.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.