AGO3 Protein, Human (sf9, His)

The AGO3 protein is critical for RNA-mediated gene silencing (RNAi) by binding to short RNAs such as microRNAs (miRNAs) and inhibiting the translation of complementary mRNAs. It is involved in stabilizing small RNA derivatives (siRNA) produced by Alu repeats and the DR2 retinoic acid response element (RARE) transcribed by processed RNA polymerase III in stem cells. AGO3 Protein, Human (sf9, His) is the recombinant human-derived AGO3 protein, expressed by Sf9 insect cells , with N-His labeled tag.

AGO3, an integral player in RNA-mediated gene silencing (RNAi), exerts its regulatory role by binding to short RNAs, including microRNAs (miRNAs), and effectively repressing the translation of mRNAs that bear complementary sequences. Its involvement extends to the stabilization of small RNA derivatives, particularly small interfering RNAs (siRNAs), originating from processed RNA polymerase III-transcribed Alu repeats that harbor a DR2 retinoic acid response element (RARE) in stem cells. AGO3 is proposed to orchestrate the subsequent siRNA-dependent degradation of a specific subset of RNA polymerase II-transcribed coding mRNAs by recruiting an mRNA decapping complex, which includes EDC4. While possessing RNA slicer activity, AGO3 exhibits this cleavage function selectively, targeting RNAs with distinct 5'- and 3'-flanking sequences within the region of guide-target complementarity.

Human

Sf9 insect cells

N-His

Q9H9G7 (M1-A860)

192669  [NCBI]

Full Length

MEIGSAGPAGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA

Approximately 90 kDa,based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.