AGO3 Protein, Human (sf9, His)
The AGO3 protein is critical for RNA-mediated gene silencing (RNAi) by binding to short RNAs such as microRNAs (miRNAs) and inhibiting the translation of complementary mRNAs. It is involved in stabilizing small RNA derivatives (siRNA) produced by Alu repeats and the DR2 retinoic acid response element (RARE) transcribed by processed RNA polymerase III in stem cells. AGO3 Protein, Human (sf9, His) is the recombinant human-derived AGO3 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The AGO3 protein is critical for RNA-mediated gene silencing (RNAi) by binding to short RNAs such as microRNAs (miRNAs) and inhibiting the translation of complementary mRNAs. It is involved in stabilizing small RNA derivatives (siRNA) produced by Alu repeats and the DR2 retinoic acid response element (RARE) transcribed by processed RNA polymerase III in stem cells. AGO3 Protein, Human (sf9, His) is the recombinant human-derived AGO3 protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
AGO3, an integral player in RNA-mediated gene silencing (RNAi), exerts its regulatory role by binding to short RNAs, including microRNAs (miRNAs), and effectively repressing the translation of mRNAs that bear complementary sequences. Its involvement extends to the stabilization of small RNA derivatives, particularly small interfering RNAs (siRNAs), originating from processed RNA polymerase III-transcribed Alu repeats that harbor a DR2 retinoic acid response element (RARE) in stem cells. AGO3 is proposed to orchestrate the subsequent siRNA-dependent degradation of a specific subset of RNA polymerase II-transcribed coding mRNAs by recruiting an mRNA decapping complex, which includes EDC4. While possessing RNA slicer activity, AGO3 exhibits this cleavage function selectively, targeting RNAs with distinct 5'- and 3'-flanking sequences within the region of guide-target complementarity.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-His
-
Accession
Q9H9G7 (M1-A860)
-
Molecular Construction
-
N-term
-
His
-
AGO3 (M1-A860)
Accession # Q9H9G7 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
AGO3; Eukaryotic Translation Initiation Factor 2C 3; Prev. EIF2C3; Argonaute 3; HAGO3; Argonaute3; Eukaryotic Translation Initiation Factor 2C, 3; EIF-2C 3; Protein Argonaute-3; EIF2C 3; FLJ12765; HAgo3; Eukaryotic Translation Initiation Factor 2C, 3, Iso
-
AA Sequence
MEIGSAGPAGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA
-
Predicted Molecular Mass
99.6 kDa
-
Molecular Weight
Approximately 90 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
